摘要
探讨不同浓度维生素C和培养时间对小鼠体细胞核移植胚胎发育潜能的影响。不同浓度维生素C(0、50、100mg/L)分别处理小鼠核移植重构胚,50 mg/L维生素C浓度组处理重构胚18 h,获得的囊胚率以及胚胎着床率显著高于其他组(P<0.05),且在此基础上提高维生素C浓度或处理时间不能进一步提高克隆胚的发育潜能(P>0.05)。小鼠体细胞核移植胚胎培养时,适当的维生素C浓度和培养时间可显著提高小鼠重构胚的发育潜能。
To investigate the impact of different concentrations and time of vitamin C on mouse somatic cell nuclear transfer embryo development potential. Mice reconstructed embryos were treated with different concentrations （0, 50, 100 mg/L） of vitamin C, the blastocyst and implantation rate was significantly higher than the other groups when this embryos were treated with 50 mg/L concentrations of vitamin C for 18 h （ P〈0.05 ） , on the basis of this condition,developmental potential of cloned embryos could not be further enhanced either by increasing the concen-tration of vitamin C or by prolonging the processing time （ P〉0.05 ） . Appropriate concentrations and incubation time of vitamin C could significantly improve the developmental potential of reconstructed embryos in the process of mouse somatic cell nuclear transfer.
出处
《安徽医科大学学报》
CAS
北大核心
2014年第5期679-681,共3页
Acta Universitatis Medicinalis Anhui
基金
安徽医科大学第一附属医院青年培育基金(编号:3101005002061)
国家自然科学基金面上项目(编号:81370691)
关键词
维生素C
体细胞
核移植
胚胎
发育潜能
vitamin C
somatic cell
nuclear transfer
embryo
development potential
