摘要
目的:明确miR-223在胶质瘤细胞中对PAX6基因3'-UTR的靶向调控作用。方法:生物信息学软件预测PAX6基因3'-UTR的靶向miRNAs;分别构建野生型和突变型PAX6基因3'-UTR双荧光素酶报告基因质粒;共转染miR-223 mimics与野生型和突变型双荧光素酶报告基因质粒于U251细胞中,双荧光素酶检测系统测定荧光素酶活性。结果:生物信息学软件预测显示PAX6可能是miR-223的靶基因;与转染野生型PAX6 3'-UTRpsiCHECKTM-2质粒组和转染突变型PAX6 Mut 3'-UTR-psiCHECKTM-2质粒组相比,miR-223 mimics能明显降低野生型荧光素酶质粒活性。结论:miR-223能够靶向负性调控PAX6基因3'-UTR的活性。
Objective：To identify the regulation of miR-223 to PAX6 gene 3′-UTR in glioblastoma cells.Methods：Bioinformatics software was applied to predict the PAX6 3′-UTR targeting miRNAs.The wild type and mutation type of PAX6 gene 3′-UTR luciferase vectors were constructed respectively.The dual luciferase assay system was used to detect the reporter activity followed by co-transfecting the luciferase vector constructed and miR-223 mimics into U25 1 cells. Results：Bioinformatics software prediction results showed that PAX6 may be a target of miR-223.The luciferase assay re-vealed that miR-223 mimics could significantly decrease the luciferase activity of wild type PAX6 3′-UTR plasmid,com-pared with the wild type and mutation plasmid transfection group,respectively.Conclusion：miR-223 could negatively target and regulate the activity of PAX6 gene 3′-UTR.
出处
《激光生物学报》
CAS
CSCD
2014年第1期59-64,共6页
Acta Laser Biology Sinica
基金
国家自然科学基金(30770838)
