摘要
为提高反应系统中的底物浓度,减少酶用量,以蒸汽爆破预处理的玉米秸秆及高性能的酵母菌株Y5进行补料同步糖化发酵研究。通过同步糖化发酵策略的优化,经两次补料使反应体系底物浓度提高至25.7%,同步糖化发酵72h,即可获得41.7g/L的乙醇浓度,综合转化率64%。同时,纤维素酶及β-葡萄糖苷酶用量分别可降至7FPU/(g纤维素)及7IU/(g纤维素)。
In order to increase the substrate loading and reduce the enzyme dosage, the fed-batch SSF process was conducted to produce ethanol from steam-exploded corn stalk by a high performance yeast strain Saccharomyces eerevisiae Y5. With optimized fed-batch SSF strategy, ethanol concentration of 41.7g/L was obtained, corresponding to an overall conversion of 64% in 72h. A high substrate loading of 25.7% （w/v） was achieved by twice feeding procedure. Simultaneously, cellulase and β-glucosidase loading can be reduced to 7FPU/g cellulose and 7IU/g cellulose, respectively.
出处
《太阳能学报》
EI
CAS
CSCD
北大核心
2014年第4期698-702,共5页
Acta Energiae Solaris Sinica
基金
国家科技支撑计划(2013BAD22B03)
国家自然科学基金(31100578)
