摘要
目的利用HPLC-DAD法建立苦碟子中木犀草素7-O-β-D-葡萄糖苷(LGCOP)、木犀草素7-O-β-D-葡萄糖醛酸苷(LGCRP)和芹菜素7-O-β-D-葡萄糖醛酸苷(AGCRP)的测定方法。方法 Diamonsil C18色谱柱(200 mm×4.6 mm,5μm);流动相为(0.05%甲酸–水)–(0.05%甲酸–乙腈);二元梯度洗脱:检测波长340 nm;体积流量1.0 mL/min;柱温35℃;进样量10μL。结果在选定色谱条件下线性关系良好(r≥0.999 9)。平均回收率分别为100.5%、100.1%、100.8%,RSD值分别为0.4%、0.3%、0.7%。结论该分析方法能简便、快速地测定苦碟子中黄酮类成分,可为评价不同产地、药用部位及采收期的药材提供科学依据。
Objective To establish an analysis method for simultaneous determination of luteolin7-O-β-D-glucopyranoside （LGCOP）, luteolin7-O-β-D-glucuronopyranoside （LGCRP） and apigenin7-O-β-D-glucuronopyranoside （AGCRP） in lxeris sonchifolia by HPLC-DAD. Methods HPLC was carried out on a Diamonsil Ci8 （ 200 mm ×4.6mm, 5 μm） column with （0.05% formic acid- water） - （0.05% formic acid - acetonitrile） as mobile phase for two elements of gradient elution. The detection wavelength was set at 340 nm. The injection volume was 10 μL at the flow rate of 1.0 mL/min. The temperature of column was set at 35 ℃. Results There were good linear relationship ofLGCOP, LGCRP, and AGCRP （r≥0.999 9）. The average recoveries were 100.5%, 100.1%, and 100.8% with RSD values of 0.4%, 0.3%, and 0.7%, respectively. Conclusion The method is convenient, specific, and can be used for the quality control of flavonoids in L sonchifolia from different areas, various medicinal parts, and different harvest.
出处
《现代药物与临床》
CAS
2014年第4期377-380,共4页
Drugs & Clinic
基金
国家科技重大专项(2011ZX09401-305-42)
国家自然科学基金青年基金项目(81102732)
