摘要
目的:内源性硫化氢(H2S)是新发现的气体信号分子,对于平滑肌细胞的舒张具有重要的调节功能。本文探讨糖尿病(DM)大鼠阴茎海绵体组织H2S信号通路及其与DM勃起功能障碍(ED)的关系。方法:8周龄健康雄性SD大鼠20只,随机分成4组:正常对照4周组(A)、DM 4周组(B)、正常对照6周组(C)、DM 6周组(D)。采用腹腔注射链脲佐菌素(50 mg/kg)制作DM模型,对照组给予等量生理盐水腹腔注射。测定各组大鼠阴茎海绵体内压/平均颈动脉压(ICPmax/MAP),取阴茎标本测定组织内H2S浓度、免疫组化和Western印迹检测阴茎海绵体组织内胱硫醚-β-合成酶(CBS)和胱硫醚-γ-裂解酶(CSE)蛋白含量。结果:电压5V和7 V刺激盆神经节测定ICPmax/MAP比值,B组(0.19±0.03,0.29±0.04)较A组(0.46±0.07,0.68±0.09),D组(0.14±0.04,0.25±0.04)较C组(0.43±0.07,0.65±0.16)均显著降低(P<0.05)。各组大鼠血清睾酮水平比较,B组[(359.08±60.06)ng/dl]与A组[(469.19±126.46)ng/dl]比较无显著差异性(P>0.05),D组血清睾酮水平[(297.01±96.58)ng/dl]与C组[(470.44±209.28)ng/dl]比较无显著差异性(P>0.05)。血浆及阴茎组织内H2S浓度,B组较A组、D组较C组均显著降低(P均<0.05)。阴茎海绵体组织内CBS和CSE蛋白表达量,B组较A组、D组较C组均显著降低(P<0.05),D组较B组亦显著降低(P<0.05)。结论:DM大鼠阴茎海绵体内H2S浓度降低、CBS和CSE表达下降,提示H2S信号系统可能与DM的ED病理机制相关。
Objective： To investigate the impact of hyperglycemia on the hydrogen sulfide （H2S） signaling pathway in rat penile tissue and its relationship with erectile function. Methods： Twenty healthy male Sprague Dawley （SD） rats aged 8 weeks were randomly divided into groups A （4-week healthy control）, B （4-week diabetes mellitus model）, C （6-week healthy control） and D （6-week diabetes mellitus model）. The rats in groups B and D were injected intraperitoneally with streptozotocin at 50 mg/kg to induce diabetes mellitus, while those in groups A and C with the same volume of normal saline. The animals were killed at 4 （ groups A and B） and 6 weeks （groups C and D） after treatment for measurement of the maximal intracavernous pressure / mean arterial blood pressure （ICPmax/MAP） by electrostimulation, determination of the H2S concentration in the plasma and penile tissue, and detection of the expressions of cystathionine-β-synthetase （CBS） and cystathionine-γ-lyase （CSE） in the penile corpus cavernosum by immunohistochemistry and Western blot. Results： With electrostimulation of the pelvic ganglia at 5V and 7 V, ICPm=/MAP was significantly reduced in groups B （0.19 ± 0.03 and 0.29 ± 0.04） and D （0.14 ± 0.04 and 0.25 ± 0.04） as compared with A （0.46 ± 0.07 and 0.68 ± 0.09） and C （0.43 ± 0.07 and 0.65 ± 0.16） （P 〈 0.05 ）. No statistically significant differences were found in the level of serum testosterone either between groups A and B （ [469.19 ± 126.46] ng/dl vs [359.08 ±60.06] ng/dl, P 〉0.05） or between C and D （ [ 470.44 ± 209.28 ] ng/dl vs [ 297.01 ± 96.58 ] ng/dl, P 〉 0.05 ）. Groups B and D showed remarkable reduction in the H2 S con- centration （P 〈 0.05） and the expressions of CBS and CSE （P 〈 0.05 ） in comparison with A and C, and the CBS and CSE expressions were even more significantly decreased in D than in B （P 〈 0.05 ）. Conclusion ： The reduced concentration of H2 S and de- creased expressions o
出处
《中华男科学杂志》
CAS
CSCD
2014年第4期299-303,共5页
National Journal of Andrology
基金
国家自然科学基金(81070486/H0415)
2010年四川省杰出青年学术技术带头人培育基金(川科技[2010]4号:2010JQ0040)~~
