摘要
目的 制备抗脆弱类杆菌和抗产气荚膜杆菌单克隆抗体池 ,并用于快速诊断及时指导临床治疗。方法 采用间接免疫荧光抗体染色法 (IFA)和免疫酶标抗体染色法 (ELA)对我院 1998～ 1999年 191例外科感染患者的标本进行细菌学检测 ,并与常规培养法进行比较。结果 3种方法从 191份标本中分别检出脆弱类杆菌 5 3株 (2 7.7% )和 5 5株 (2 8.8% )以及 2 5株 (13 .1% ) ;检出产气荚膜杆菌 12株 (6.3 % )和 11株 (5 .8% )以及 6株 (3 .1% )。IFA和ELA法 2种厌氧菌检出率明显高于CM法。但IFA和ELA法之间检出率差异无显著性。结论 自制抗脆弱类杆菌和抗产气荚膜杆菌的McAb池 ,检测平时常见的脆弱类杆菌和战时常见的产气荚膜杆菌 ,敏感性高 ,特异性强 ,简便 ,快速 ,便于推广。
Objective To prepare monoclonal antibody (McAb) pool against Bacteroides fragilis and Clostridium perfringens and evaluated its effect in rapid diagnosis in surgical infection. Methods After the preparation of monoclonal antibodies against B. fragilis and C. perfringens , 191 specimens collected from infectious patients was detected with indirect immunofluorescent antibody (IFA) and enzyme labeled antibody (ELA). The results were compared with routine cultural method (CM). Results Among the 191 specimens, 53 (27.7%), 55 (28.8%) and 25 (13.1%)strains of B. fragilis were detected with IFA, ELA and CM respectively, and for C.perfringens , 12(6.3%), 11(5.8%), and 6 (3.1%) strains were found. The detectable rate of anaerobic bacteria by IFA and ELA was higher than by CM, but no difference was found between these 2. Conclusion The self made McAb pool against B.fagilis and C.perfringens might be a rapid, specific, sensitive and simple McAb pool for detecting B.fragilis in surgical infection and C.perfringens in wartime.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第2期224-226,共3页
Journal of Third Military Medical University
基金
全军"九五"指令性课题!( 96L0 4 2 )
