摘要
目的:建立毛细管柱气相色谱法测定紫杉醇中残留的有机溶剂。方法:采用HP-INNOWAX毛细管色谱柱、顶空进样、氢火焰离子化检测器,进样口温度为250℃,检测器温度为300℃,柱温为35℃保持5 min,再以25℃·min-1升至260℃。结果:正己烷、丙酮、乙酸乙酯与甲醇相邻峰间的分离度及检测灵敏度均符合要求。结论:该法简便、快速、灵敏,结果准确可靠,可用于紫杉醇中残留有机溶剂的测定。
Objective：To establish a capillary GC method for the determination of residual organic solvents in paclitaxel. Meth-ods：The standard solution and test solution was respectively injected into an HP-INNOWAX sillica capillary column by headspace in-jection. The chromatograms were recorded with an FID. The injection port and detector temperature was 250℃ and 300℃, respective-ly, the initial column temperature was kept at 35℃ for 5 min, and then was risen to 260℃ at the rate of 25℃/min. Results：The four residual organic solvents could be separated completely. The resolution and sensitivity were acceptable. Conclusion： The method is proved to be simple, rapid, sensitive and reliable, and can be used in the determination of residual organic solvents in paclitaxel.
出处
《中国药师》
CAS
2014年第4期588-589,共2页
China Pharmacist
