摘要
目的 探讨胰腺微循环障碍在急性胰腺炎发病机制中的作用 ,研究中药 WPY的作用机理。方法 以异硫氰酸荧光素标记红细胞 ( FITC- RBC)作为示踪剂 ,采用活体荧光显微镜检术观察雨蛙素 ( caerulein)诱导建立的小鼠急性胰腺炎模型早期胰腺微循环的改变。同时以中药 WPY颗粒剂作为干预措施 ,观察了对其早期微循环改变的影响。结果 雨蛙素造模后 6小时 ,胰腺微循环中红细胞流速、功能性毛细血管密度、小叶间动脉管径均显著降低 ( P<0 .0 1)。经 WPY灌饲后 ,小叶间动脉管、功能性毛细血管密度、红细胞流速均有显著恢复 ( P<0 .0 5 )。结论 急性胰腺炎早期胰腺微循环障碍在其发病机制中起重要作用 ,可能是进一步导致胰腺出血、坏死的主要因素。中药 WPY可以显著改善急性胰腺炎病情 。
Objective To explore the pathogenesis of acute pa ncreatitis (AP) a nd evaluate the effect of a Chinese herb WPY on the course of AP. Metho ds Intra vital fluorescence microscopy was used. The pancreatic microvascular diameter, R BC velocities and functional capillary density (FCD) were estimated following in tracutaneous injection of caerulein alone or with gastropipe WPY. Result s Caerul ein mediated a significant decrease in functional capillary density (FCD), RBC v elocities and diameter of interlobular arteriole( P <0.01). Compared with AP g roup, WPY was effective in restoring functional capillary density, interlobular arteriole diameter and RBC velocity. Conclusion Impairment of p ancreatic microcirculation in the early phase of acute pancreatitis may play a k ey role in the progression of this disease. Possible contributory mechanisms inc lude reduced blood flow and functional capillary density, interlobular arteriole spasm, and leukocyte endothelial cell interaction. WPY has a beneficial effect on the course of acute pancreatitis. Possible causes include attenuating microc irculatory failure.
出处
《华西医科大学学报》
CAS
CSCD
北大核心
2001年第1期92-95,共4页
Journal of West China University of Medical Sciences
