摘要
在室内半静水的试验条件下,应用实时荧光定量PCR技术研究了不同质量浓度(0μg·L-1、5μg·L-1、10μg·L-1、50μg·L-1和100μg·L-1)的0#柴油水溶液(WSF)胁迫15 d和清洁海水恢复7 d中翡翠贻贝(Perna viridis)外套膜与内脏团组织中CYP4基因的相对表达水平变化。2-△△Ct法分析结果表明,CYP4基因在翡翠贻贝外套膜和内脏团中均有表达,WSF胁迫对其表达水平有明显的诱导作用,且表达水平具有组织差异性;内脏团表达水平明显高于外套膜,两组织CYP4基因相对表达水平随着WSF胁迫时间的延长,整体呈现出先诱导后抑制再诱导的波动变化趋势,其中50μg·L-1浓度组表现最为显著(P<0.01)。WSF胁迫解除后外套膜与内脏团CYP4基因相对表达水平迅速下降,部分浓度组逐渐恢复到正常水平。
Green mussels （Perna viridis）, exposed to 0 μg.L-1 , 5μg-L-1 , 10 μg.L-1 , 50 μg.L-1 and 100 μg.L-1 No. 0 diesel oil water-soluble fraction （WSF） for 15 d under semi-hydrostatic simulation, were transferred to clean sea water for 7 d to analyze the gene expression levels of CYP4 by the method of 2-△△ct combining with real-time quantitative PCR. Results show that WSF obviously induced CYP4 up-regulation and was differentially expressed in both visceral mass and mantle in P. viridis. The expression level of CYP4 was obviously higher in visceral mass than in mantle. The relative gene expression level initially increased then decreased and in- creased again as time elapsed in those two kinds of tissues. The most significant difference was observed in 50 μg.L-1 concentration group （ P 〈 0.01 ） compared to the control. At the last 7 d, the expression level in visceral mass and mantle decreased quickly and that in some groups gradually returned to normal level by being transferred to clean sea water.
出处
《南方水产科学》
CAS
CSCD
北大核心
2014年第2期61-66,共6页
South China Fisheries Science
基金
科技部科研院所社会公益研究专项资金项目(2005DIB3J020)
中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2007ZD003,2010TS13)
广东省重大科技计划项目(009B030600002)
国家自然科学基金项目(31100362)
