摘要
在萨氏营养液(SDB)中加入用 0.1%蔗糖脂肪酸酯-15(SE15)乳化的0.50%芝麻油,可使在SDB中生长慢而结块的新蚜虫疠霉、安徽虫瘟霉、飞虱虫疠毒和根虫瘟霉等四种虫霉的五个菌株形成均匀分散的菌丝体和能稳定转接的菌液。在SDB中加入乳化芝麻油 ( 0.25%~1.5%)、菜籽油( 0.50%~1.5%)和色拉油( 80%花生油和 20%菜籽油,0.75%~1.5%),能明显提高新蚜虫疠霉F98018的生物量;而在SDB中加入0.50%乳化芝麻油,可使液培菌丝产孢量增加近一倍;在SDB中加入的三种植物油(0.75%),在F98018生长4d后,含量分别下降了85%~94%,但其中脂肪酸相对组成基本不变,表明该菌生长能充分利用植物油中各脂肪酸。用萨氏培养基( SDAY)、加入 0.50%乳化芝麻油的 SDAY( OS-SDAY)和牛奶蛋黄培养基(SEMA)培养安徽虫瘟霉F97028和新蚜虫疠霉F98028,两菌株在SEMA上生长最快;在OS-SDAY以较慢速度正常生长;而在SDAY上,两菌株或不能生长,或在第二次培养时出现明显退化。用SEMA和OS-SDAY在3℃下来保存上述四种虫霉的七个菌株,在OS-SDAY上?
Sesame oil (0.50%) emulsified by (0.1%, W/V) Sucrose Fatty Acid Esters (SE-15), were added into Sabouraud dextrose broth (SDB), facilitating five isolates of Pandora neophidis, P. delphacis, Zoophthora anhuiensis and Z radicans to develop a homogenous mycelia unclumping and subculturable. For P. neoaphidis F98018, the biomass were obviously improved after addition of emulsified sesame oil (0.25%~1.5%), rapeseed oil (0.50%~1.5%) and salad oil (0.75%~1.5%, 80% peanut oil and 20% rapeseed oil) in SDB, and sporulation of mycelium mats was also improved by nearly 100% after addition of 0.50% emulsified sesame oil in SDB. When the concentration of sesame oil, rapeseed oil or salad oil in SDB was 0.75%, after 4 day's growth of p. neoaphidis F98018, the volume of oils decreased by 85%~94%, while the percentages of fatty acids in the oils remained constant. The result indicated the fungus could uptake all of the fatty acids in plant oils comprehensively and without any selectivity. Sabouraud dextrose agar (SDAY), SDAY added with 0.5% emulsified sesame oil (OS-SDAY), and Sabouraud egg yolk-milk agar (SEMA) were used comparatively to culture P. neoaphidis F98028 and Z anhuiensis F97028. The two he were subculturable on SEMA (most favorably) and OS-SDAY (slower than on SEMA), While on SDAY, Z anhuiensis F97028 could not grow, and P. neoaphidis F98018 showed degenerated growth at the second subculture. The media of SEMA and OS-SDAY were used to preserve seven isolates of P. neoaphidis, P. delphacis, Z anhuiensis, and Z radicans, all of the fungi on OS-SDAY remained alive after 9 months, While five isolates on SEMA died. Meanwhile P. neoaphidis F98028 and Z anhuiensis F97028 didn't show degeneration or alteration of growth and colonial morphology after 9 months of preservation on os-SDAY.
出处
《菌物系统》
CSCD
北大核心
2001年第1期79-86,共8页
Mycosystema
基金
国家杰出青年科学基金!(39525004)
国家自然科学基金!(39370479)
关键词
虫霉
植物油
液体培养
保存
虫霉菌
Entomophthoralean fungi, Plant oils, Liquid culture, Preservation