摘要
目的探讨铅及其化合物对铅蓄电池生产工人血液中氧化损伤及能量代谢指标的影响。方法首先检测铅蓄电池厂590位工人的血铅(BPb),根据BPb值选取BPb≤120μg/L的非暴露工人58人为对照组,血铅在180μg/L≤BPb≤220μg/L水平的51位工人为低暴露组,血铅在280μg/L≤BPb≤320μg/L范围的52位工人为中暴露组,BPb≥380μg/L的55位为高暴露组,检测血清中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性来评价氧化损伤程度,检测血清中琥珀酸脱氢酶(SDH)及全血总ATP酶活性来评价铅对能量代谢的影响。结果低、中、高三个暴露组血清SOD活性要比对照组低,差异有统计学意义(P<0.05),中暴露组和高暴露组男性工作者血清SDH活性比对照组高,差异有统计学意义(P<0.05),而女性工作者四个组间SDH活性差异无统计学意义(P>0.05)。不论男性还是女性,血清MDA含量和全血总ATPase酶活性四组间差别无统计学意义(P>0.05)。结论铅可使血清SOD活性降低,血清SOD活性是较MDA更敏感的评价铅氧化损伤的指标;铅可使男性暴露者血清SDH活性升高,提示这种影响可能存在性别差异。
Objective To investigate effects of lead and its compounds on biomarkers of oxidative damage and energy me- tabolism in the blood of battery workers. Methods First, blood lead （BPb） of 590 workers in the lead battery factory were detected, and then according to BPb values ,58 workers whose BPb were not more than 120 p^g/L were selected as the control group in the non - exposed workers,51 workers with blood lead from 180 p.g / L to 220 p,g / L were as low - exposure group, 52 workers with blood lead from 280 p.g / L to 320ug / L were as the intermediate exposure group and 55 workers with BPb not less than 380 p^g / L were as the high exposure group. The level of malondialdehyde （MDA） and the activity of superoxide dismutase （SOD） in serum were tested to evaluate the degree of oxidative damage. Activities of succinate dehydrogenase （SDH） in serum and total ATPase in whole blood were detected to evaluate the effects of lead on energy metabolism. Results Compared with the control group, the activity of SOD in low, medium and high exposure groups had significantly declined （ P 〈 0.05 ） ; The level of SDH in serum of medium and high exposure groups were higher than that of the control group, which only was seen in male workers（P 〈0.05）and was not reflected in female workers（P 〉 0.05） ; Whether male or female, the levels of MDA in serum and total ATPase in whole blood did not have statistically significant difference among the four groups （ P 〉 0.05 ）. Conclusion Lead could reduce the activity of SOD and the activity of SOD was a indicator more sensitive than MDA on about assessment of lead oxidative damage ; lead bring about the rise about of the activity of SDH in serum to male workers, suggesting that this effect may have gender differences.
出处
《中国辐射卫生》
2014年第2期181-184,共4页
Chinese Journal of Radiological Health
基金
山东省安全生产科技发展计划项目(LAK2012-10)
关键词
铅
氧化损伤
能量代谢
Lead
Oxidative Damage
Energy Metabolism
