摘要
目的:了解大黄素等中药提取物对幽门螺杆菌(Helicobacter pylori,H.pylori)克拉霉素耐药基因的作用,探索可以有效抑制耐药性H.pylori的方法.方法:采用对倍稀释法检测大黄素等对克拉霉素耐药的H.pylori最低抑菌浓度(minimum inhibitory concentration,MIC),在中草药提取物1/2MIC环境下威迫培养耐药性H.pylori,5 d传代1次,续传6次;E-test药敏实验检测威迫培养前后克拉霉素对耐药性H.pylori的MIC提取H.pylori DNA,DNA测序法检测耐药基因,对比前后突变.结果:大黄素等中药提取物能降低克拉霉素对耐药H.pylori的MIC,对克拉霉素耐药的H.pylori均出现23s rRNA基因的A2143G、A2144G点突变;黄连素等中药提取物作用30d后未发现基因有回复突变.结论:大黄素等中药提取物对克拉霉素耐药的H.pylori有一定的协同抑制作用,协同作用与H.pylori 23s rRNA耐药基因回复突变无关.
AIM： To evaluate the effects of emodin and other herbal extracts on resistance of Helicobacter pylori （H. pylori） to clarithromycin, and to explore themethods for effectively inhibiting H. pylori anti- biotic resistance. METHODS： The MIC of herbal extracts against clarithromycin resistance of H. pylori was detect- ed by double dilution method. Clarithromycin resistant H. pylori was cultured in medium con- taining herbal extracts at a concentration of half MIC and passaged once every 5 d for 6 times. The MIC of clarithromycin against antibiotic resistance of H. pylori was assessed before and after stress culture. The DNA of H. pylori was extracted to detect drug resistant-related gene mutations. RESULTS： The MIC of clarithromycin against H. pylori was reduced by herbal extracts. The gene mutations of 23s rRNA A2143G and A2144G were detected in clarithromycin-resistant H. pylori. No back mutations were discovered after treatment with herbal extracts for 30 d. CONCLUSION： Herbal extracts have a syner- gistic effect in inhibiting the clarithromycin re- sistance of H. pylori. The synergistic action is not associated with the back mutations of 23s rRNA gene mutations related to clarithromycin resis- tance of H. pylori.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第6期825-830,共6页
World Chinese Journal of Digestology
基金
广西教育厅科研立项基金资助项目
No.桂教科研[2010]10号
广西高校优秀人才资助计划基金资助项目
No.桂教人[2011]40号
广西自然科学基金资助项目
No.桂财教[2012]53号
2012GXNSFAA053172~~
关键词
中药提取物
幽门螺杆菌
耐药基因
克拉
霉素
Herbal extracts
He/icobacter pylori
Drug resistance-related gene
Clarithromycin
