摘要
目的对国产的5种抗-HIV检测试剂进行质量比较。方法采用抗-HIV质控血清盘、标准浓度为2NCU/mL的质控血清及常规的血液样品对5种国产的ELISA试剂进行灵敏度、特异性、精密性、最低检测限、板间变异系数及反应模式的均一性等参数分析。结果抗-HIV的ELISA检测试剂质量参数存在差异;常规血液样品S/CO结果分布显示反应模式的均一性有差别。结论血液筛查实验室对抗-HIV ELISA试剂的选择,应该在参照参考品的考核结果的同时,结合试剂盒本底均一性等综合因素进行。
Objective To evaluate the quality of 5 types of HIV antibody reagents. Methods The quality parameters such as sensitivity, specificity, precision, minimum detection limit, inter-plate coefficient of variation and uniformity of re-sponse mode were analyzed by using anti-HIV control serum plate,2 NCU/mL quality control serum and routine blood sam-ples. Results The quality parameters of HIV antibody ELISA reagent are different. The S/CO of routine blood samples showed that there were differences in the reaction modes. Conclusion Blood screening laboratory for anti-HIV ELISA rea-gent selection, in accordance with the reference material results, it should be combined with kit background homogeneity fac-tors at the same time.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2014年第3期276-278,共3页
Chinese Journal of Blood Transfusion
基金
四川卫生厅课题(120501)
