摘要
目的 探索建立颅咽管瘤体外模型的方法 ,寻找博莱霉素治疗颅咽管瘤的理论依据。方法 取 5人份新鲜颅咽管瘤组织块 ,以胶原酶消化成单细胞悬液 ,进行体外培养 ,在不同时段计数以画出生长曲线 ;加入 0 0 1mg/ml,0 1mg/ml和 1mg/ml等含博莱霉素的不同浓度培养液培养 ,在不同时段计数以画出抑瘤曲线 ,并取加药培养 6天后细胞以ATP荧光测定法测定抑瘤效果。结果 4人份组织块短期培养成功 ,细胞数随时间延长而下降 ,加药后下降更明显 ,且浓度越高下降越明显 ,ATP荧光测定法显示 ,0 1mg/ml和 1mg/ml浓度组有显著抑瘤效应 ,而 0 0 1mg/ml组则耐药。结论 以消化法可成功进行颅咽瘤短期体外培养 ,并用于建立体外模型 ,博莱霉素可抑制颅咽管瘤体外细胞 ,存在浓度和时间依赖效应 ,ATP荧光测定法用于药敏实验敏感、准确 。
Objective To investigate a methods to construct the model of craniopharyngiomas in vitro and the theoretic data of Bleomycin on this tumor. Methods 5 fresh tissues from 5 different craniopharyngiomas were obtained to be digested and then cultured in vitro. The growing cure line was drawn on the cell numbers in certain time point. The different concentrations bleomycin containing culture fluids were added into the well growing cells instead of the culture fluids. The inhibitory tumor cure line was drawn based on the calculating cell numbers. The ATP was extracted out of the cells by TCA methods after 6 days and assessed by ATP luminescence assay. Results 4 tissues were successfully cultured and the cell number decreased with time. When Bleomycin added, the cell number decreased more and the decreased rate related to the drug concentration. With ATP luminescence assay, all of the drug added groups except for the 0 01mg/ml group showed marked inhibitory effects. Conclusions Craniopharyngiomas may be primaryly cultured in vitro for short term as the model in vitro by digesting methods. Its cells in vitro may be inhibited by Bleomycin and its inhibitory effect relies on the concentration of the drug and the time. When used in drug inhibitory test, ATP luminescence assay is suscepitivity and dependable.
出处
《中华神经外科杂志》
CSCD
北大核心
2001年第1期29-31,共3页
Chinese Journal of Neurosurgery
