摘要
目的 探讨辅助T细胞17（Th17）相关细胞因子[白细胞介素（IL）-23、17A、21、22、6,转化生长因子-β（TGF-β）]在复发缓解型多发性硬化（RRMS）发病机制中的作用及甲泼尼龙对其影响.方法 设立RRMS组38例和神经系统非炎性疾病（NNID）患者20例作为对照组,ELISA方法检测NNID患者和甲泼尼龙治疗RRMS患者前后血清和脑脊液中IL-23、IL-17A、IL-21、IL-22、TGF-β、IL-6的水平.结果 RRMS组治疗后血清和脑脊液中IL-17A、IL-23、IL-21、IL-22水平较治疗前明显下降,但仍较NNID组高,TGF-β水平较治疗前增高[血清：（17.0±5.9） pg/ml与（34.1±6.5）pg/ml,t=14.351,P=0.000；脑脊液：（26.4 ±4.7） pg/ml与（73.2±19.7） pg/ml,t=16.352,P=0.000];RRMS组治疗前血清和脑脊液中TGF-β水平低于NNID组[血清：（30.2±8.9） pg/ml,t=6.769,P=0.012;脑脊液：（31.4±7.5） pg/ml,t=9.368,P=0.017],治疗后脑脊液中TGF-β水平显著高于NNID组（t=9.138,P=0.000）；相关性分析：RRMS组治疗前血清中IL-23与IL-17A正相关；脑脊液中IL-23、IL-17A、IL-21两两正相关.结论 甲泼尼龙治疗后RRMS血清和脑脊液中IL-23、IL-17A、IL-21、IL-22水平降低,TGF-β水平升高.IL-23、IL-17A、IL-21、IL-22、TGF-β与RRMS发病可能相关.
Objective To investigate the effect of methylprednisolone on T helper 17 cell （Th17 cells） related cytokines （interleukin （IL）-23,17A,21,22,6,and tansforming growth factor （TGF）-β） in serum and cerebrospinal fluid from patients with relapsing remitting multiple sclerosis and their effects on the pathogenesis.Methods We recruited relapsing remitting multiple sclerosis group （38 patients）and noninflammatory neurological disease group （20 controls）,and detected the levels of IL-23,IL-17A,IL-21,IL-22,TGF-β and IL-6 in serum and cerebrospinal fluid （CSF） with ELISA kit in both controls and patients before and after treatment by methylprednisolone.Results After treatment in relapsing remitting multiple sclerosis patients,IL-17A,IL-23,IL-21,and IL-22 levels in cerebrospinal fluid and serum were significantly decreased,however,they were still higher than that in the non-inflammatory neurological disease patients.TGF-β levels was significantly increased （serum：（17.2 ± 5.9） pg/ml vs （34.1 ± 6.5） pg/ml,t =14.351,P =0.000 ; CSF：（26.4 ± 4.7） pg/ml vs （73.2 ± 19.7） pg/ml,t =16.352,P =0.000）.The levels of TGF-β in serum and CSF in patients before treatment were lower than those of in non-inflammatory neurological disease patients （serum：（30.2 ± 8.9） pg/ml,t =6.769,P =0.012 ; CSF：（3 1.4 ± 7.5） pg/ml,t =9.368,P =0.017）.However,the levels of TGF-β in CSF in patients after treatment were significantly higher than those in non-inflammatory neurological disease patients （t =9.138,P =0.000）.Correlation analysis showed that IL-23 and IL-17A were positive correlation in the serum of relapsing remitting multiple sclerosis patients before treatment.Moreover,positive correlations among IL-23,IL-17A and IL-21 were detected in the CSF of relapsing remitting multiple sclerosis patients before treatment.Conclusions Decreased levels of IL-23,IL-17A,IL-21 and IL-22,and elevated levels of TGF-β were detected in serum and CSF of patients with relapsing remitting mu
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2014年第4期246-249,共4页
Chinese Journal of Neurology
