摘要
采用静水试验方法,研究了Cd2+对青鱼的急性毒性效应以及Cd2+安全浓度胁迫对青鱼鳃丝、肌肉和肝脏的超氧化物歧化酶(SOD)和金属硫蛋白(MT)的影响。结果表明:Cd2+对青鱼的96 h LC50为1.434 mg/L,Cd2+对青鱼的生态毒性为高毒。在0.143 mg/L(安全浓度)Cd2+胁迫下,各组织中SOD活性和MT含量在6 h、12 h内显著上升,呈现出一定时间-效应关系,以暴露6 h时最为显著(P<0.05);暴露12 h后,MT的含量基本上趋于平衡,而SOD活性却是快速下降,表明了12 h后该浓度Cd2+污染胁迫对SOD活性的抑制作用。整个试验期间,对照组青鱼各组织中的SOD活性和MT含量大小顺序皆为肝脏>鳃丝>肌肉,同种组织内相对比较稳定;肌肉中的SOD活性要显著低于鳃丝和肝脏,而肝脏中的MT含量要显著高于肌肉和鳃丝。
Acute toxicity of waterborne Cd 2+to Mylopharyngodon piceus and the effects of low concentration of Cd 2+on Superoxide Dismutase(SOD)activities and Matellothionein(MT) content in gill, muscle, and liver of Mylopha-ryngodon piceus were investigated with static exposure techniques .The results showed that 96 h LC50 of Cd2+to My-lopharyngodon piceus was 1.434 mg/L with high eco-toxicity.SOD activities and MT contents in tissues at safe con-centration of 0.143 mg/L Cd2+increased significantly within 6 hours and 12 hours ,which showed dramatically time effect relationships and the maximal inducing effect was observed within 6 hours(P<0.05).After 12 hours, MT contents were comparatively stable , indicating inducing mechanism was close to the thresholds .In contrast , the SOD activities decreased rapidly after 12 hours , which was caused by the inhibitory effects of Cd 2 +.The activities of SOD and the contents of MT in tissues showed the same trends:liver>gill>muscle .The activity of SOD in mus-cle was significantly lower than those in gill and liver , while the MT content in liver was significantly higher than those in muscle and gill .
出处
《水生态学杂志》
北大核心
2014年第2期90-95,共6页
Journal of Hydroecology
基金
浙江省科技计划项目(2011C23062)
