摘要
目的:测定人工种植红景天不同药用部位花瓣、茎、根的含量。方法:取各1 g样品经过60%的乙醇溶解,超声波清洗器清洗50 min,过滤,旋蒸,加2 mL的甲醇,用微孔滤膜(0.45μm)过滤,采用CAPCELL PAK C18柱(2.0 mm×150mm,i.d.,3μm),柱温30℃,波长278 nm;样品温度10℃,进样体积10μL。流动相为甲醇-1%乙酸溶液45:55,流速为1 mL·min-1进行定量分析。结果:有效部位中的红景天苷含量从高到低依次为花瓣(35.00mg)、根(18.89mg)、茎(1.59 mg)。结论:建立了红景天中红景天苷含量的高效液相色谱方法,可适用于不同药材中红景天苷含量的测定。
To determine the petals,stems,roots planted content Rhodiola different medicinal parts.Method：Each 1 g sample is dissolved after 60％ ethanol,ultrasonic cleaner cleaning 50 min,filtered,rotary evaporated,add 2 mL of methanol,microporous filter membrane （0.45 μm） filter using CAPCELL PAK C18 column （（2.0 mm× 150 mm,i.d.,3 μm）,column temperature 30 ℃,wavelength 278 nm,sample temperature 10 ℃,injection volume of 10 μL.The mobile phase was methanol-1％ acetic acid solution 45∶55,flow rate of 1 mL · min-1 for quantitative analysis.Result：The effective parts of salidroside content in the order of the petals （35.00 mg）,root （18.89 mg）,stems （1.59 mg）.Conclusion：A detection of salidroside content in Rhodiola rosea was established by high performance liquid chromatography.It can be applied to the analysis on the salidroside in the different medicinal materials.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第8期79-82,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家公益性行业科技专项项目(201303040-13)
"十二五"农村领域国家科技计划项目(2011BAD34B03)
