摘要
目的研究并建立枳实药材的高效液相色谱特征图谱。方法采用色谱柱Hypersil ODS(4.6 mm×200 mm,5μm),以乙腈(A)-水(B)为流动相系统进行梯度洗脱;柱温35℃;检测波长284 nm;流速1.0 mL·min-1。结果通过对10批不同产地的枳实药材的测定,建立了枳实药材的指纹图谱共有模式,标定了2个共有峰,以化学对照品结合高效液相色谱图鉴定了这2个峰。10批枳实药材的相似度均在0.90以上。结论该方法简便,准确,重现性好,为有效控制药材的内在质量标准提供科学依据。
Objective To study and establish the fingerprint chromatogram for the identification of citrus aurantium.Methods A HPLC analysis was carried out on a Hypersil ODS （4.6 mm＆#215;200 mm, 5μm） with the mobile phase consisting of acetonitrile （A）-water （B） in gradient mode.The detection wavelength was set at 284 nm and the column temperature was kept at 35 ℃.The flow rate was 1.0 mL·183; min -1 . Results The mutual mode of the fingerprints of Citrus aurantium was set up with “Similarity Evaluation System for Chromatographic Fingerprint ofTCM ”, and two characteristic peaks were marked, which were identified by combination of high performance liquid chromatogram and chemical reference sample.The similaritiesof 10 batches of citrus aurantium were above 0. 90.Con clusion The method proves to be repeatable, precise and stable.It can provide scientific basisfor the quality control of citrus aurantium.
出处
《辽宁医学院学报》
CAS
2014年第2期3-5,I0002,I0003,共5页
Journal of Liaoning Medical University (LNMU) Bimonthly
基金
辽宁省教育厅科研项目
项目编号:L2010332
