摘要
目的观察肿瘤坏死因子(TNF-α)对急性肺损伤动物模型外周血骨髓间充质干细胞(MSCs)数量的影响。方法 SD大鼠分为对照组、模型组和干预组。对照组给予生理盐水气道滴入;模型组给予内毒素(LP S 5 mg/kg)气道内滴入;干预组气道内滴入LPS同时英夫利昔(10 mg/mL)腹腔注射5 mg/kg。5 h后处死动物取外周血,检测TNF-α含量及成纤维细胞集落形成单位(CFU-F)数。成骨、成脂诱导检测其分化特性。结果模型组大鼠外周血TNF-α水平较对照组显著升高(354.0±82.4 vs 38.0±21.6,P<0.05),而较干预组显著降低(206.5±64.1 vs 354.0±82.4,P<0.05)。模型组大鼠外周血中培养出的CFU-Fs明显高于对照组(3.60±1.47vs 1.40±2.83,P<0.05)及干预组(3.60±1.47 vs 1.70±1.72,P<0.05);外周血骨髓MSCs成骨、成脂诱导后染色均阳性。结论急性肺损伤时,外周血中增加的TNF-α含量对骨髓MSCs动员及归巢均有促进作用。
[Objective] To evaluate the effects of TNF- a on Mobilization of Mesenchymal Stem Cells （MSCs） on acute lung injury in the peripheral blood （PB） of rats. [Methods] 24 male and female SD rats were randomly divided into three groups. Control group （CG group, n =6） were intratracheal instillation and subcutaneously injected with sodium chloride. Model of acute lung injury （MG group, n =8） were intratraeheal instillation of lipopolysaceharide （LPS 5 mg/kg） and subcutaneously injected with sodium chloride. Interfering group （IG group, n =8） were subcutaneously injected with infliximab after intratracheal instillation of LPS. 5 hour after intratracheal instillation, PB was collected from heart of rats in each group. The density of TNF- a in PB was detect, MSCs were harvested from PB by density gradient centrifugation. The number of the colony forming unit-fibroblasts （CFU- Fs） was counted when peripheral blood mononuclear cells were cultured at 14 d. MSCs were analyzed with muhipotent differentiation, which was identified through osteogenic and adipogenic induction. [Results] The LPS caused significant increasing in blood serum TNF- a level in model group in comparison with control group （354.0 ± 82.4 vs 38.0± 21.6, P 〈0.05） and decreased in interfering group in comparison with model group （206.5 ± 64.1 vs 354.0 ± 82.4, P〈0.05）. The results indicated that the number of CFU- F in model group was more than that in control group （3.6± 1.47 vs 1.4± 2.83, P〈O.05）, the number of CFU - F in interfering group was decreased compared with model group （1.7 ± 1.72 vs 3.6 ± 1.47, P 〈0.05）. 28 days after osteogenic induction of MSCs, calcium deposition was detected by Alizarin Red, 21 days afteradipogenic differentiation,lipid droplets were detected by oil red. [Conclusion] TNF- a has the imobilization effect in MSCs derived from bone marrow in rat model of ALI.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2014年第5期17-21,共5页
China Journal of Modern Medicine
基金
2010年陕西省卫生厅科研资助项目(No:2010D38)
关键词
急性肺损伤
骨髓间充质干细胞
外周血
acute lung injury
bone marrow mesenchymal stem cells
peripheral blood
