摘要
目的:研究平喘宁对寒性哮喘大鼠气道重塑及其肺组织中ERK2 mRNA及c-fos mRNA表达水平的影响。方法:雄性SD大鼠70只,随机分为正常组、模型组、地塞米松组、桂龙咳喘宁组以及平喘宁高、中、低剂量组,每组为10只。以卵蛋白致敏及寒冷刺激复制大鼠哮喘模型,造模21d后,正常组、模型组每天给予等量蒸馏水灌服,地塞米松组、桂龙咳喘宁组、平喘宁高、中、低剂量组按人体与大鼠体表面积换算比折算剂量灌胃,灌胃4周后解剖大鼠并取出肺组织。采用HE染色法观察肺组织的病理形态学改变;采用逆转录-聚合酶链式反应以半定量测定ERK2 mRNA、c-fos mRNA的表达丰度。结果:模型组发生明显肺组织炎症反应和气道重塑现象,地塞米松组、桂龙咳喘宁组、平喘宁组较模型组有不同程度的好转;模型组ERK2 mRNA、c-fos mRNA表达水平比正常组显著升高(P<0.01,P<0.05);经平喘宁小、中、大剂量治疗后,ERK2 mRNA、c-fos mRNA表达水平减低(P<0.05,P<0.01)。结论:平喘宁明显减轻哮喘大鼠气道炎症反应,抑制哮喘大鼠肺组织中ERK2 mRNA、c-fos mRNA的表达,延缓气道重塑而治疗哮喘。
Objective： To investigate the effect of Pingchuanning on expression of ERK2 mRNA and c-fos mRNA in Lung tissues of asthma rats. Methods： 70 male SD rats were randomly divided into the control group, model group, dexamethasone group, Guilongkechuanning group and Pingch uanning low, middle, high-dose group, with 10 in each group. The asthma model was established by ovalbumin sensitized and cold stimulate on rats. After 21 days of the model establishment, the rats in normal and model control groups were gavaged with the same amount of distilled water every day. The dexamethasone group, Guilong Kechuanning group and high, medium and low dose of Pingchuanning groups were gavaged with drug drench according to the ratio of human to rat body surface area. After 4 weeks of treatment, the rats were dissected, and their lung organs were taken out. The lung histopathology changes were observed by HE staining. The expression of ERK2 mRNA and c-fos mRNA in the lungs were detected by RT-PCR. Results： The rats in model group had obvious lung organ inflammation and airway remodeling. Compared with the model group, the rats in the treatment group had better changes in varying degrees; The ERK2 mRNA and c-fos mRNA in asthma model group were increased than the control group （P〈0.01, P〈0.05）; After the treatments of low, middle and high-does of Pingchuanning, the ERK2 mRNA and c-fos mRNA were reduced （P〈0.01, P〈0.05）. Conclusion： These results show that Pingchuanning might effectively alleviate the airway inflammation and reverses the airway remodeling by inhibiting the expressions of ERK2 mRNA and c-fos mRNA,
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2014年第4期1221-1224,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金(No.81173187)
安徽省自然科学基金(No.11040606M217)~~
