摘要
目的：观察心脏骤停复苏后（ CA-CPR）心功能和心肌κ阿片受体（κ-OR）表达的变化及参附注射液对其影响，探讨参附注射液保护复苏后心功能的新机制。方法健康雄性SD大鼠，随机分为健康对照组（BL）、参附注射液组（SF）和对照组（CON）。所有大鼠均气管插管，经颈动脉左心室插管和股动脉插管监测血流动力学参数，经股静脉置管建立给药通道。采用气管夹闭窒息法制作心脏骤停动物模型。 BL组不复制模型，SF组和CON组窒息5 min后予心肺复苏（CPR）。 SF组CPR同时静脉泵注参附注射液20 mL/kg（60 mL/h），CON组泵注等量生理盐水。动态记录HR、MAP、dP/dt40、LVEDP和-dP/dtmax等血流动力学参数。拟定复苏后0．5、2、4、6、24 h五个时间点随机处死大鼠并取心肌组织备检，每个时间点均取满6只。 RT-PCR法测定心肌κ-OR mRNA表达，Western blot法测定心肌κ-OR蛋白含量。结果 CON和SF两组复苏成功率、窒息至心跳停搏时间（ Tc）、窒息至呼吸停止时间（ Tb）、开始心肺复苏至恢复自主循环时间（Tr）比较差异无统计学意义（P＞0．05）； CON组与SF组复苏后24 h生存率比较差异无统计学意义（χ2＝1．25，P＝0．26）。 BL组血流动力学参数始终保持在基线水平，SF组复苏后五个时间点HR、MAP、dP/dt40及-dP/dtmax均显著优于CON组（P＜0．01），且6 h后除-dP/dtmax始终低于BL组（P＜0.01）外，SF组HR、MAP与BL组比较差异无统计学意义（P＞0．05）。复苏大鼠心肌中κ-OR mRNA表达水平和κ-OR蛋白含量均呈先升后降曲线，均以复苏后2 h表达量最高，而BL组两者水平均显著低于CON组和SF组（P＜0．05）；与SF组比较，CON组心肌中к-OR mRNA和к-OR蛋白含量显著升高（ P＜0．05）。结论 CA-CPR对心肌κ-OR mRNA表达水平及κ-OR蛋白含量均有显著影响，参附注射液可以改善复苏后血流动力学参数，对复苏后心�
Objective To investigate the relationship between cardiac function and expression of kappa-opioid receptor （κ-OR） in a cardiac arrest cardiopulmonary resuscitation （ CA-CPR） rat model and the intervention effects of Shengfu Injection （ SFI） on them, and to explore a new mechanism of SFI in decreasing post -resuscitation myocardial dysfunction .Methods Cleaning grade Sprague -Dawley rats were divided into three groups randomly named Baseline Control Group （ BL ） , SFI Intervention Group （ SF ） and Experimental Control Group （ CON ） . All rats were undergone oral intubation, polyethylene catheter advanced into left ventricle （LV） via surgical exposure of right carotid artery for measurement of LV pressure and another polyethylene catheter from the left femoral artery to descending aorta for aortic pressure and arterial blood samples , an additional polyethylene catheter was＆amp;nbsp;cannulated into the left external femoral vein for medication infusion .Asphyxia -induced CA -CPR model was performed by turning -off the ventilator and clamping the endotracheal tube to lead cardiac arrest and carried out chest compression and mechanical ventilation for CPR after 5 min of asphyxia .BL rats didn＆#39;t perform CA-CPR.SF and CON rats were infused with 20 mL/kg （60 mL/h） SFI or 20 mL/kg （60 mL/h） saline at the same time of CPR, respectively.HR, MAP, dP/dt40, LVEDP and -dP/dtmax were measured dramatically .Six rats for each time interval （ 0.5, 2, 4, 6, 24 h post -resuscitation） in each group were sacrificed and the hearts were harvested .The mRNA and protein impressions of κ-OR in post-resuscitation myocardium were measured by methods of RT -PCR and Western blot .Results There were no differences of CPR success rate and time from asphyxia to cardiac arrest （ Tc） and time from asphyxia to breath suspending （ Tb ） and time from CPR to restoration of spontaneous circulation （Tc） between CON group and SF group （P＆gt;0.05）.The 24 h survival rate was not different b
出处
《中国急救医学》
CAS
CSCD
北大核心
2014年第4期353-357,共5页
Chinese Journal of Critical Care Medicine
基金
国家自然科学基金青年项目（81201445）
