摘要
选择体重１５．１８±０．１０ｋｇ的三元杂交（杜×长×大）去势公猪９头．按体重随机分为３个处理，分别饲喂以下日粮：①２０％蛋白质日粮（异亮氨酸含量０．６４％，血球粉用量４．５％），即２０％ＣＰ组；②低蛋白质（１２％）日粮补充赖氨酸、蛋氨酸、苏氨酸和色氨酸（异亮氨酸含量０．４１％，血球粉用量６．０％），即１６％ＣＰ组：③在第２种日粮的基础上进一步补充０．２３％异亮氨酸，即１６％ＣＰ－Ｉｌｅ组。试验共进行两期。通过１５Ｎ－甘氨酸标记及终产物分析法测定仔猪全身蛋白质周转。结果表明．在各必需氨基酸含量及其组成模式基本相同的条件下．将日粮蛋白质水平白２０％降至１６％，未影响仔猪全身蛋白质合成速度、全身蛋白质降解速度及蛋白质沉积速度（Ｐ＞０．１０）·但氨代谢池中有较小比例的氮被氧化（Ｐ＜０．０５）。异亮氨酸“缺乏”日粮中补充异亮至氨并使其“满足”仔猪的需要以改善日粮蛋白质质量，显著提高了仔猪金身蛋白质合成速度（Ｐ＜０．０５）和全身蛋白质降解速度（Ｐ＜０．０７），且全身蛋白质合咸速度的增加值高于全身蛋白质降解速度的增加值，最终导致蛋白质沉积速度的显著增加（Ｐ＜０．０５）。
Nine weanling barrows (Duro×Landrace×Yorkshire, 15. 18(0.10kg) were selected to investigate the effects of crystal isoleucine supplementation (0. 23%) of a low protein (16%) and low isoleucine (0. 41%) diet supplemented with lysine, methionine, threonine and tryptophan on whole-body protein turnover. The pigs were allotted randomly to each of dietary treatments: (1) 20% crude protein diet containing 0. 64% of isoleucine (group 20%CP); (2) Low protein (16%) diet supplemented with lysine, methionine, threonine and tryptophan, contain 0.41% of isoleucine (group 16%CP). (3) Isoleucine-supplemented (0. 23%) low protein (16%) diet with the supplementation of lysine, methionine, threonine and tryptophan (group 16%CP+Ile). The experiment lasted two periods. The results showed that no significant differences in whole-body protein synthesis rate, whole-body protein breakdown rate and protein accretion rate were found between Group 20%CP and Group 16%±Ile (P>0. 10). Whole-body protein synthesis rate (P< 0.05), whole-body protein breakdown rate(P<0. 07) and protein accretion rate (P<0..05) in Group 16%CP-Ile were significantly increased when compared with those in Group 16%CP.
出处
《中国农业科技导报》
CAS
CSCD
2001年第1期38-42,共5页
Journal of Agricultural Science and Technology
