摘要
目的 建立同时测定一清软胶囊中8种有效成分含量的方法.方法 采用Xtimate-C 18色谱柱,0.2％磷酸水（A）和乙腈（B）梯度洗脱,流速0.6 ml/min,检测波长280 nm,柱温30℃.结果 盐酸小檗碱、盐酸黄连碱、黄芩素、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚分别在进样量范围内与峰面积线性关系良好,r＞ 0.9997,仪器精密度和稳定性RSD均＜2％,各成分的回收率在98％～103％ （n=3）.测定了3批一清软胶囊中8种指标成分的含量.结论 建立的方法可同时测定一清软胶囊中8种有效成分的含量,方法简单、重复性好.
Objective To establish a method for simultaneous quantitative determination of eight active components in Yiqing Soft capsule.Methods The separation was performed on Xtimate-C 18 chromatographic column with mobile phase consisted of water （containing 0.2％ phosphoric acid）-acetonitrile （gradient elution） at a flow rate of 0.6 ml/min.Detecting wavelength was set at 280 nm and the column temperature was set at 30℃.Results The calibration curve of berberine,coptisine-hydrochloride,baicalein,aloe-emodin,rhein,emodin,chrysophanol and physcion showed good linear regression in test ranges （r ＞ 0.9995）; and the overall recoveries were in the range of 97％-103％ （n=3）.The overall RSD of precision test were less than 2％.Conclusion The extracting method is convenient and credible,and the quantitative method is accurate and repeatable,and it can be used in simultaneous quantitative determination of eight active components in Yiqing soft capsule.
出处
《国际医药卫生导报》
2014年第7期889-892,共4页
International Medicine and Health Guidance News
基金
国家自然科学基金项目（81073045）
