摘要
目的探讨不同病程慢性乙肝(CHB)患者血清白细胞介素-32(IL-32)、总胆红素(TBIL)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)及HBV DNA载量中的差异及其相关性。方法选取2011年1月至2013年5月收治的105例慢性CHB患者(轻度组32例、中度组42例、重度组31例)为研究对象,另选取100例健康体检者为对照组,两组血清IL-32采用酶联免疫法测定,TBIL、ALT、AST采用采用日立7600-020全自动生化分析仪测定,HBV DNA载量采用荧光定量PCR测定。结果与对照组相比,CHB组血清IL-32、TBIL、ALT、AST及HBV DNA载量显著提高,差异有统计学差异(P<0.05)。与轻、中度组相比,重度HCB组血清IL-32、TBIL、ALT、AST及HBV DNA载量显著提高,差异有统计学意义(P<0.05)。HBV DNA高载量组(>105拷贝数/ml)血清IL-32、TBIL、ALT、AST水平显著高于HBVDNA低载量组(<105拷贝数/ml),差异有统计学意义(P<0.05)。结论血清IL-32可能介导CHB炎症反应,参与CHB病情的发生及进展,血清IL-32及肝功能指标可作为CHB患者病情进展的预测指标。
Objective To investigate the levels of serum interleukin -32 （IL -32）, total bilirubin （TBIL）, alanine aminotransferase （ALT）, aspartate aminotransferase （AST） and HBV DNA of course of chronic hepatitis B （CHB） and their relationship. Methods 105 patients with chronic CHB patients （ 32 patients with mild, moderate group 42 cases , 31 cases of severe group） were selected in the study. At the same time 100 patients healthy were selected for the control group from January 2011 to December 2013. The levels of IL - 32 were determined by enzyme - linked immunosorbent assay. The levels of TBIL, ALT, AST were determined by Hitachi 7600 - 020 automatic biochemica. The load of HBV DNA were measured by quantitative PCR. Results The levels of serum IL - 32, TBIL, ALT, AST and HBV DNA of CHB groups were increased than those of control group （ P 〈0.05 ）. The levels of serum IL - 32, TBIL, ALT, AST and HBV DNA of severe HCB groups were increased than those of mild and moderate group （ P 〈 0.05 ）. The levels of serum IL - 32, TBIL, ALT, AST of High HBV DNA load group （ 〉 105 copy num- ber/ml） were higher than those of HBVDNA low - load group （ 〈 105 copy number/ml） （ P 〈0.05 ） . Conclusion The levels of serum IL - 32 may mediate inflammation CHB. CHB involved in the occurrence and progress of the disease, serum IL - 32 can be used as indicators of liver function predictors of disease progression in patients with CHB .
出处
《临床和实验医学杂志》
2014年第6期478-481,共4页
Journal of Clinical and Experimental Medicine
