摘要
目的:检测支气管哮喘患者外周血中血清白细胞介素33(IL-33)的水平,探讨其与哮喘患者肺功能、免疫球蛋白E(IgE)的关系及其在支气管哮喘发病机制中的作用。方法:选择50例支气管哮喘患者作为实验组,均行肺功能及IgE水平检测,依据血清IgE水平将哮喘患者分为IgE升高组(n=25)和IgE正常组(n=25),另外选取20名健康对照者作为健康对照组,采用ELISA法检测受试者外周血中血清IL-33水平,比较健康者和哮喘患者急性发作期及临床控制期血清IL-33水平、第1秒用力呼气容积(FEV1)和呼气峰流速(PEF);分析IL-33水平与肺功能和IgE水平的相关性。结果:支气管哮喘急性发作期患者血清IL-33水平明显高于健康对照组(P<0.05);IgE升高组患者血清IL-33高于IgE正常组(P<0.05);临床控制期患者血清IL-33水平低于急性发作期者(P<0.05),但高于健康对照组(P<0.05)。哮喘急性发作期患者血清IL-33与患者FEV1和PEF均呈明显负相关关系(r=-0.413,P=0.003;r=-0.504,P<0.001);哮喘临床控制期患者血清IL-33水平与患者FEV1和PEF亦呈负相关关系(r=-0.386,P=0.006;r=-0.324,P=0.022)。结论:IL-33作为一种炎性因子参与了哮喘的免疫-炎症反应机制,并可能加剧哮喘患者肺功能恶化。
Objective To investigate the role of interleukin-33 （IL-33） in the pathogenesis of bronchial asthma through measuring the serum IL-33 levels in the perpheraal blood of the patients with bronchial asthma, and to study the relationship between the IL-33 levels and lung function or immunoglobulin E（IgE） in the patients with bronchial asthma. Methods Fifty patients with bronchial asthma （experiment group） and twenty healthy controls （healthy control group） were enrolled in the study. The asthma patients were divided into IgE increased group （n= 25） and IgE normal group （n= 25）according to the IgE level. The serum IL-33 levels in peripheral blood of the subjects were examined by ELISA method. The IL-33 level, forced expiratory volume in one second（FEV1）, and peak expiratory flow（PEF） were compared among asthma patients at acute attack period or clinical control period and healthy controls. The correlation between the serum IL-33 level and lung function or IgE level was further analyzed. Results The serum IL-33 level in asthma patients at acute attack period was higher than that in healthy control group（P〈0.05） ; the IL-33 level of the patients in IgE increased group was higher than that in IgE normal group（P〈0.05） ; the serum IL-33 level of the patients at clinical control period was lower than that of the patients at acute attack period（P〈0.05）, but it was still higher than that of healthy controls（P〈0.05）. The serum IL-33 level of the asthma patients at acute attack period showed significantly aegative correlation with FEV1 and PEF of the patients（r=-0. 413, P= 0. 003; r=-0. 504, P〈0. 001） ; the serum IL-33 level of the asthma patients at clinical control preriod also showed negative correlation with FEV1 and PEF of the patients （r =- 0. 386, P = 0.006; r=-0.324, P=0.022）. Conclusion IL-33, as an inflammatory factor, participates in the immune-inflammatory response of bronchial asthma, and may aggravate the lung function of asthma patients.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2014年第2期395-398,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科研基金资助课题(3D511Y833428)
吉林大学研究生创新基金项目资助课题(20121121)
