摘要
目的 探讨急性羰基镍染毒大鼠血清镍水平的变化规律,为临床救治急性羰基镍中毒患者提供实验室支持依据.方法 以羰基镍250 mg./in3、500 mg/m3两个剂量组给予SPF级大鼠各54只静态吸入30 min,分别于染毒后30 min、2h、4h、8h、12h、24 h、48 h、72 h、7d将大鼠以乙醚在15 s内麻醉,立即剖腹暴露腹主动脉,采集2 ～3 mL血液,分离0.5 ～1 mL血清,用美国PE公司AA800原子吸收仪（石墨炉法）检测血清镍含量,并与健康大鼠相比较.结果 250 mg/m3剂量组30 min、2h、4h、8 h、12 h、24h、48 h、72 h、7d血清镍含量平均值分别为（33.69±2.59） μg/L、（24.61 ±3.03） μg/L、（27.83±5.69）μg/L、（21.36±4.14）μg/L、（20.39 ±4.14） μg/L、（18.80±7.02）μg/L、（14.51±8.21） μg/L、（13.58±5.78）μg/L、（12.83±4.41）μg/L,30 min为峰值,为对照组的5.30倍,各时间段与健康大鼠差异均有统计学意义（t值分别为5.959、5.958、5.990、5.998、5.997、5.994、5.990、4.317、4.347,均P＜0.01）.500 mg/m3剂量组30 min、2h、4h、8h、12 h、24h、48 h、72 h、7d血清镍含量平均值分别为（72.22±1.62） μg/L、（57.78±12.99） μg/L、（42.25±7.25）μg/L、（103.77±11.ll）μg/L、（79.04±12.26） μg/L、（26.35±6.56）μg/L、（18.58±4.92）μg/L、（17.22±9.73） μg/L、（14.59±5.27） μg/L,8h为峰值,为健康大鼠的16.33倍,各时间段与健康大鼠差异均有统计学意义（t值分别为5.960、5.947、5.978、5.927、5.948、5.959、3.143、2.447、2.440,均P＜0.05）.两组30 min、2h、4h、8h、12h等时间段差异均有统计学意义（t值分别为5.208、2.447、2.449、5.959、5.959,P值分别为0.001、0.049、0.042、0.000、0.000）.结论 急性羰基镍染毒大鼠血清镍含量与染毒剂量呈明显的剂量-效应关系;羰基镍或其代谢产物的排出主要在24h内.
Objective To master the serum nickel change rule of acute nickel carbonyl poisoning in rats, and to provide laboratory support for clinical treatment of acute nickel carbonyl poisoning patients. Methods SPF rats were given nickel carbonyl（250mg/m3,500mg/m3） in static inhalation for 30min. Rats were anesthetized by ether for 15 seconds after exposure for 30min,2h,4h, 8h, 12h,24h,48h, 72h and 7d respectively. Anatomized rats, kept blood collection 2-3mL, and separated serum 0.5-1mL. Serum nickel was detected by AA800 （American PE company）. Results The average serum nickel levels of 250mg/m3 dose group at 30min,2h,4h,8h,12h,24h,48h,72h,7d were （33.69 ±2.59） ,（24.61 ±3.03） ,（27.83 ±5.69） ,（21.36±4.14） ,（20.39 ±4.14） ,（18.80±7.02） ,（14. 51 ± 8.21 ）, （ 13.58 ± 5.78 ） and （ 12.83_± 4.41 ） Ixg/L. 30 minutes reached the peak, and was 5.30-fold of those in nor- mal controls. There had significant differences compared with normal control（ t = 5. 959,5. 958,5. 990,5. 998,5. 997, 5. 994,5. 990,4.317,4. 347 ,all P 〈 0.01 ）. The average serum nickel levels of 500mg/m3 dose group at 30min,2h, 4h ,8h, 12h ,24h ,48h, 72h, 7d were （72.22 ± 1.62 ）, （57.78 ± 12.99 ）, （42.25 ± 7.25 ）, （103.77 ± 11.11 ）, （79.04 ± 12.26）, （26.35 ± 6.56 ） , （ 18.58 ± 4.92 ）, （ 17.22 ± 9.73 ）, （ 14.59 ±5.27 ） μg/L. 8h reached the peak,and was 16.33-fold of normal controls. The differences were significant （ t = 5. 960,5. 947,5. 978,5. 927, 5. 948,5. 959,3. 143,2. 447,2. 440, all P 〈 0.05 ）. Meanwhile, there were significant differences between two groups of 30min,2h,4h,8h,12h（t = 5. 208,2. 447,2. 449,5. 959,5. 959,P = 0. 001,0. 049,0. 042, 0. 000,0. 000）, but there was no significant difference after 24h. Conclusion There was significant doses-effect relationship between ser- um nickel content of acute nickel carbonyl poisoning rats and the dosage. Nickel carbonyl or its metabolites were excreted mainly within 24h.
出处
《中国基层医药》
CAS
2014年第6期818-819,共2页
Chinese Journal of Primary Medicine and Pharmacy
基金
甘肃省金川集团有限公司与兰州大学产学研合作项目（2009-12）
关键词
羰基镍
血清镍
Carbonyl nickel
Serum nickel
