摘要
目的:观察HeLa细胞感染柯萨奇病毒B3(CVB3)后Bax和Bim mRNA和蛋白的表达。方法:将HeLa细胞分为未予以CVB3感染的对照组和予以CVB3感染的病毒组,取3 h、6 h、9 h、12 h和24 h的细胞,采用RT-PCR和Western blotting分别检测Bax和Bim mRNA和蛋白的表达。结果:Bax和Bim mRNA在对照组和病毒组HeLa细胞中均有表达,在病毒组和对照组中Bax和Bim mRNA的表达在各自组内均无显著差异(P>0.05),但在24 h Bax和Bim在病毒组mRNA的表达低于对照组(P<0.05)。Bax和Bim蛋白表达在对照组中各时点均有表达,但无显著差异(P>0.05)。病毒组Bax蛋白在24 h表达下降,差异有统计学意义(P<0.05),且病毒组表达明显低于对照组(P<0.05);Bim蛋白的表达随感染时间延长而下降(P<0.05),9 h、12 h和24 h表达病毒组低于对照组(P<0.05)。结论:Bax和Bim在CVB3感染诱导的HeLa细胞凋亡早期发挥作用。
AIM： To observe the expression of Bax and Bim at mRNA and protein levels in coxsackievirus B3 （CVB3）-infected HeLa ceils. METHODS： HeLa cells were divided into control （non-CVB3 infection） group and CVB3 infection group. The expression of Bim and Bax at mRNA and protein levels was determined by reverse transcriptase poly merase chain reaction （RT-PCR） and Western blotting analysis at 3 h, 6 h, 9 h, 12 h and 24 h postinfection. RE SULTS ： The mRNA expression of Bax and Bim in control group was not significantly different among different time points, so was that in CVB3 infection group （ all P 〉 0. 05）. However, compared with control group, the mRNA expression of Bax and Bim in CVB3 infection groups was decreased at 24 h postinfection （ all P 〈 0. 05 ）. The protein expression of Bax and Bim in control group was not significantly different among different time points （ P 〉 0. 05 ）. However, in CVB3 infection group, the protein expression of Bax was lower at 24 h postinfection than that at 3 h （P 〈 0. 05）. Meanwhile, compared with control group, the protein expression of Bax in CVB3 infection group was decreased at 24 h postinfection （ P 〈 0. 05 ）. The protein expression of Bim in CVB3 infection groups was decreased along with the time postinfection, and was decreased at 9 h, 12 h and 24 h as compared with control group （P 〈0. 05）. CONCLUSION： The proteins of Bax and Bim play an important role in CVB3-induced apoptosis of HeLa cells.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2014年第3期524-528,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30973233)
中南大学创新课题资助项目(No.2011ssxt196)
