摘要
目的检测血浆和外周血单个核细胞（PBMC）中EB病毒（epsteinbarrvirus，EBV）与人巨细胞病毒（humancyto—megalovirUs，HCMV）DNA含量，以期为两种病原体的早期诊断与监测提供敏感、准确、及时的实验室结果，并为两种病原体感染的实验室诊断筛选出适宜的标本类型。方法采用实时荧光定量聚合酶链反应（realtime—PCR）分别对270份血浆和外周血单个核细胞中EB痛毒和HCMV病毒进行核酸检测并分析。结果270份血浆和外周血单个核细胞中EBVDNA检出率分别是30％和96．7％，血浆和外周血单个核细胞两种类型标本检测EBVDNA的联合检出率为100％；血浆和外周血单个核细胞两种类型标本对HCMVDNA检出率分别是40％和86．7％，两种类型标本对HCMVDNA的联合检出率为100％。结论外周血单个核细胞标本对EB病毒和HCMV的检出率明显高于血浆标本，血浆和单个核细胞两种类型标本对EBVDNA和HcMVDNA的联合检出率为100％。临床医生在疑似EBV和HCMV感染患者的诊疗中要选择适宜的标本类型；首选外周血单个核细胞标本进行检测，条件允许的情况下与血浆标本联合检测，以提高检测的敏感性和准确性。
Objective In order to supply a mdi＇e aeetlrate, sensitive and early reference results for clinical diagnosis and treat- ment; Epstein-barr virus DNA and human cytotnegalo virus DNA were detected with real time fluorescence quantitative pol- ymerase chain reaction using two kind sattlples of plasma and peripheral blood mononuclear cells respectively. Methods U sing real-time fluorescence quantitative pcllymerase chain reaction, Epstein-barr virus DNA and human cytomegalo virus DNA from 270 viruses infected plasmas and peripheral blood mononuclear cells were detected. Results The Epstein-barr vi- rus DNA detection rate of plasmas and peripheral blood mononuclear cells were 30% and 96.7%. Combined 2 kind samples of plasma -and peripheral blood mononuclear cells with a high detection rate was 100%. The Human cytomegalo virus DNA detection rate of plasmas and peripheral blood monotmelear cells were 40 % and 86.7 %. Combined 2 kind samples of plasma arid peripheral blood mononuclear cells with a high detection rate was 100%. Conclusion The DNA detection ratio of pe- ripheral blood mononuclear cells was significantly higher than in plasma. Combined 2 kind samples of plasma and peripheral blood mononuclear cells with a high detection rate was 100%. It is necessary choosing a correct sample type in diagnosis of virus related infection diseases. Sample of peripheral blood motlonucleat cells was first choosing. Combination of plasma and peripheral blood mononuelear cells would have a good sensitivity and accurate results.
出处
《现代检验医学杂志》
CAS
2014年第1期105-107,共3页
Journal of Modern Laboratory Medicine
关键词
实时荧光定量聚合酶链反应
血浆
外周血单个核细胞
EB病毒
人巨细胞病毒
fluorescence quantitative polymerase chain feaetiom plasma
peripheral blood mononuclear cells epstein-barr vi-rus
human cytomegalo virus
