摘要
目的探讨雷帕霉素对急性髓系白血病(AML)细胞株Kasumi-1mTOR/p70S6K通路的影响。方法免疫荧光检测雷帕霉素处理前后Kasumi-1细胞中mTOR和p70S6K的光密度变化,再逆转录-PCR、Western blot分别从RNA和蛋白水平研究雷帕霉素对该信号通路的影响。结果经雷帕霉素处理后mTOR的mRNA和蛋白水平均下调,而其下游靶点p70S6K的mRNA和蛋白水平表达增加。结论雷帕霉素对细胞增殖和凋亡产生的影响可能是通过mTOR/p70S6K信号通路发生作用。
Objective To study the effect of rapamycin on mTOR/p70S6K pathway in human acute myeloid leukemia（AML） cell line Kasumi-1. Methods The change of optical density of mTOR/p70S6K before and after the rapamycin treatment was detec-ted by the immunofluoreseence method. Then the effect of rapamycin on the signal pathway was studied from the RNA and protein level by the reverse transcription-PCR and Western blot. Results The mRNA and protein expressions of mTOR were down-regula-ted after rapamycin treatment. However mRNA and the protein level of the downstream target p70S6K of mTOR was increased. Conclusion Rapamycin could play the effect on the cell proliferation and apoptosis by the mTOR/p70S6K pathway.
出处
《国际检验医学杂志》
CAS
2014年第6期657-658,共2页
International Journal of Laboratory Medicine
关键词
雷帕霉素
急性髓系白血病
聚合酶链反应
rapamycin
acute myelogenous leukemia
polymerase chain reaction
