摘要
目的观察雷公藤内酯对老年大鼠术后认知功能障碍海马TNF-α表达的影响。方法将入选大鼠随机分为3组:即假手术组(sham组),模型组(POCD组)和给药组(TP组)。实验组术前1 d、手术当日和术后1 d腹腔注射TP 0.4 mg·kg-1,共3次。肝脏部分切除术后1、7 d时各取7只大鼠,采用Y型迷宫检测术后第1、2、3、7、8、9天时学习记忆能力。术后第3天时各取7只大鼠海马进行免疫组织化学染色及图像分析,测定TNF-α阳性细胞数和平均光密度。结果与模型组比较,雷公藤内酯给药组大鼠术后8 d全天总反应时间明显缩短(P<0.05),术后2、3、7、8、9 d错误反映次数明显减少(P<0.05),术后2、3、7、8 d主动回避次数明显增加(P<0.05)。给药组大鼠海马区TNF-α阳性细胞数和平均光密度明显降低(P<0.05)。结论雷公藤内酯明显减轻肝部分切除术后大鼠认知功能障碍,其机制可能与其通过抑制炎症反应,减少TNF-α表达有关。
Objective To observe the influence of tripterygium glycosides on expression of TNF-α in hippocampus of elderly rats suffering from postoperative cognitive dysfunction. Methods 48 rats were enrolled and randomly assigned into three groups： sham group（ n = 14）; model group（ n = 21）,suffering from partial hepatectomy under anaesthesia with pentobarbital sodium; TP group,administered with tripterygium glycosides 0. 4 mg·kg-1per day during perioperative period. On the postoperative 1 d and 7 d,7 rats from each group were respectively assessed in the ability of learning and memory with Y-maze. 7 rats from each group were detected of expression of TNF-α in hippocampus on the postoperative 3 d through immunohistochemistry. Results Compared with model group,TP group’s total reaction time shortened statistically on the postoperative 8 d（ P ＆lt; 0. 05）,error number reduced statistically on the postoperative 2 d,3 d,7 d,8 d and 9 d（ P ＆lt; 0. 05）. Active avoidence times increased statistically on the postoperative 2 d,3 d,7 d and 8 d（ P ＆lt; 0. 05）. The TNF-α positive cell number and average optical density in hippocampus decreased significantly（ P ＆lt; 0. 05） compared to model group. Conclusions Tripterygium glycosides relieved elderly rats’ postoperative cognitive dysfunction after partial hepatectomy. The potential mechanism perhaps is inhibition of inflammatory reaction by decreasing TNF-α expression.
出处
《安徽医药》
CAS
2014年第2期239-241,共3页
Anhui Medical and Pharmaceutical Journal
