摘要
目的探讨JSH-23阻断NF-κB信号通路后人舌鳞癌细胞Tca8113增殖和凋亡的情况及NF-κB信号通路相关凋亡抑制基因Bcl-2的表达变化,进而为临床舌鳞癌的干预和治疗寻找新的药物提供实验依据。方法采用体外培养人舌癌Tca8113细胞的方法,经不同浓度JSH-23作用不同时间后,用MTT法、RT-PCR及Western blot等方法检测JSH-23对Tca8113细胞增殖、凋亡的影响。采用SPSS13.0统计软件对数据进行方差分析及t检验。结果 Tca8113细胞经JSH-23作用后,增殖受到明显抑制,20μmol/L JSH-23作用48 h时,抑制作用最为显著,细胞生长抑制率达到60.45%。同时,Bcl-2凋亡抑制基因表达减少,与对照组相比,JSH-23作用48 h后,Bcl-2表达显著减少47.85%,差异有统计学意义(P<0.05);Bcl-2蛋白含量也明显减少,作用48 h后,Bcl-2蛋白表达相对光密度值分别下降50.93%(P<0.01)。结论 JSH-23作为NF-κB信号通路抑制剂,可以明显抑制人舌鳞癌细胞的增殖,同时显著下调Bcl-2基因及蛋白的表达。
Objective To explore the effect of the NF-KB signaling inhibitor JSH-23 on proliferation and apoptosis of Tca8113 cells for providing an experimental basis in treatment of tongue squamous cell carcinoma. Methods The apoptosis and proliferation of Tca$113 cells were detected using MTF, RT-PCR and Western blot after incubation with different concentrations of JSH-23 for different time. SPSS13.0 statistical software was used for data analysis of variance and t test. Results The proliferative activity of Tca8113 cells after treated with JSH-23 was inhibited obviously, expecially at 20 ~mol/L for 48 h（ the inhibitive rate of 60.45% ）. And com- pared with controls, Bcl-2 gene expression decreased after coculatured with JSH-23, expecially for 48 h, the Bcl-2 gene expression de- creased by 47.85 % significantly （ P 〈 0.05 ）, and the Bcl-2 protein significantly decreased by 50.93 % （ P 〈 0.01 ）. Conclusion JSH-23, as a NF-kappa B signaling pathway inhibitor, can significantly inhibit the proliferation of human tongue squamous cancer cells and downregulate the Bcl-2 gene and protein expression.
出处
《山西医科大学学报》
CAS
2014年第3期186-189,共4页
Journal of Shanxi Medical University
基金
唐山市科技局课题基金资助项目(13130265a)
