摘要
为了查明浙江省温州市瑞安大面积杨梅枝条枯死的主要原因,采用组织分离法对枯死的杨梅枝条进行病原分离,获得1种主要真菌分离物HN?01。根据分离物的培养性状、孢子大小,结合显微镜检测和分子生物学ITs序列测定,该菌株被鉴定为聚多拟盘多毛孢(Pestalotiopsis sydowiana)。对菌株进行柯赫氏法则验证,证明聚多拟盘多毛孢是导致杨梅枝条枯死的病原菌。筛选4种杀菌剂对该病原菌进行室内毒力测定,结果 50%咪鲜胺可湿性粉剂的防效较好,EC50值为1.21 mg/L。
To find out the main cause of dead branches ofMyrica rubra Sieb.et Zucc at Wenzhou ruian, Zhejiang province, tissue isolation was conducted on dead branches and one fungal isolate HN-01 was separated. According to the morphological characteristics, spore size, microscopy and the sequence of the internal transcribed spacer （ITs）, the strain HN-01 was identified as Pestalotiopsis sydowiana. Based the rule of Koch＇s postulation, P sydowiana was proved to be the pathogen causing the exsciccation of myrica sp. branches. Four major fungicides were screened against the pathogen in laboratory and 50% prochloraz WP had good control effect with 50% effective concentration value of 1.21 mg/L.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第1期53-55,共3页
Journal of Hunan Agricultural University(Natural Sciences)
基金
温州市科技局项目(N20100001)
关键词
杨梅
拟盘多毛孢
ITS序列
室内毒力
Myrica rubra Sieb.et Zucc
Pestalotiopsis sydowiana
identification
ITs sequence
indoor toxicity
