摘要
目的探讨胸苷酸合成酶(TS)、核苷酸切除修复交叉互补基因1(ERCC1)及拓扑异构酶-Ⅱ(TopoⅡ)在结直肠癌中的表达及临床意义。方法选择本院2011年5月—2013年5月接受结直肠癌根治性手术的90例患者,应用SP免疫组化联合检测TS、ERCC1及TopoⅡ在结直肠癌组织和对照组20例正常结直肠组织中的表达,并结合临床病理因素进行分析。结果①TS、ERCC1在结直肠癌组织中的表达高于正常结直肠黏膜组织,TopoⅡ在结直肠癌组织中低表达(P<0.05);②TS表达与年龄有关,在≥60岁患者组中表达低于<60岁组;③ERCC1表达与不同病理类型有关,而与肿瘤分化程度及淋巴结转移无关;④TopoⅡ表达与结直肠癌的病理类型及分化程度均有关(P<0.05),而与浸润深度及淋巴结转移无关。结论 TS、ERCC1及TopoⅡ均在结直肠癌对抗肿瘤药物耐药中起重要作用。联合检测TS、ERCC1及TopoⅡ对于结直肠癌判断预后、制定化疗方案有一定的指导意义。
Objective To explore the expressions and clinical significance of thymidylate synthase（TS）, nucleotide excision repair cross-complement genel （ERCC1） and topoismerase-II （Topo II ） in colorectal carcinoma. Methods Both expressions of TS,ERCCland Topo II in 90 pa- tients with rectal carcinoma and expression of 20 normal tissues in control group were detected by S -P immunohistochemical technique, and its correlation with clinical pathologic characteristics of car- cinoma was analyzed as well. Results （1） The expression rates of TS, ERCClin carcinoma of the colon and rectum were significantly higher than those in normal tissues （P 〈 0.05）, while the ex- pression rate of Topo II in carcinoma of the colon and rectum was significantly lower （P 〈 0.05）. （2） The expression rate of TS was closely related with age, which was higher in the old than the young. （3） The expression rate of ERCC1 was significantly related with pathological types, while was signif- icantly unrelated with the tumor differentiation and lymphatic metastasis. （4） The expression rate of Topo II was significantly related with pathological types and tumor differentiation, while was signif- icantly unrelated with lymphatic metastasis and depth of tumor invasion. （5） There was no correla- tion between every two indexes among the expression levels of TS, ERCC1 and Topo II. Conclusion TS, ERCC1 and Topo IIplay an important role in the chemoresistance of the colorectal carcino- ma. Determination of TS, ERCC1 and Topo II may be useful for the estimate of prognosis and the establishment of chemical therapy regimen in colorectal carcinoma.
出处
《实用临床医药杂志》
CAS
2014年第3期98-99,102,共3页
Journal of Clinical Medicine in Practice
