摘要
目的从毒性病理学角度了解双酚A(BPA)对小鼠睾丸组织病理损伤的作用,为研究BPA毒性作用提供形态学依据。方法选用108只SPF雄性CD-1小鼠,称重后随机分为4个剂量组(0、100、300、600 mg/kg BW)。BPA经口染毒8周,取睾丸组织固定,HE及免疫组化染色后进行光镜和电镜观察,并进行半定量统计。结果 BPA染毒8周后与对照组比较,BPA 300、600 mg/kg BW组小鼠体重降低,睾丸指数呈现下降趋势。光镜观察发现BPA染毒小鼠睾丸曲精小管支持细胞、各级生精细胞变性坏死、脱落,精子数量减少;透射电镜观察发现BPA引起睾丸生精细胞出现核固缩、顶体囊和顶体帽发育不良或发育畸形,基膜增厚。免疫组化检测结果表明,与对照组比较,BPA染毒组小鼠睾丸组织NF-κB表达量明显升高(P<0.01);BPA 300、600 mg/kg BW剂量组Caspase-3表达量明显升高(P<0.01)。TUNEL检测表明BPA染毒组小鼠睾丸凋亡阳性信号与对照组比较差异有统计学意义(P<0.01)。结论上述结果表明经口染毒不同剂量BPA对小鼠睾丸组织均产生了不同程度的损伤,诱导睾丸组织细胞凋亡。
Objective In order to investigate the effects of bisphenol A （BPA） on testicle tissue of mice from toxicologic pathological perspective, and providd morphological evidences of BPA toxicity. Methods 108 SPF CD-1 male mice were randomly allocated into four groups with BPA concentration of O, 100, 300 and 600 mg/kg BW. All BPA groups were administrated orally with different doses for 8 weeks continuously. Then testicles were taken and fixed to perform pathological observation and analysis. Results After 8 weeks of BPA administration, body weight and testicle index of BPA groups were decreased compared to control group. Degeneration and necrosis were observed in sustentacular cell of seminiferous tubule, and sl^ermatozoon number decreased. Karyopyknosis and maldevelopment were occurred at acrosome vesicle and cap of spermatogenic cells observed by TEM. Immunohistochemistry test showed that NF-KB and Caspase-3 expression were up-regulated （P 〈 0. 01 or P 〈 0. 05 ） in BPA groups compared to control group. TUNEL test indicated that the number of apoptosis positive cells was higher in BPA groups than control group （P 〈 O. O1 ）. Conclusion Different doses of BPA could lead to testicle tissue damage at different level, and induce cell apoptosis of testicle.
出处
《中国食品卫生杂志》
北大核心
2014年第2期127-132,共6页
Chinese Journal of Food Hygiene
基金
教育部长江学者和创新团队发展计划支持项目(IRT0866)
高等学校博士学科点专项科研基金(20B0008110030)
关键词
双酚A
小鼠
睾丸
经口毒性
病理损伤
细胞凋亡
食品污染物
毒理学实验
Bisphenol A
mice
testicle tissue
oral toxicity
pathological injury
apoptosis
food contaminant
toxicological experiment
