摘要
为了开发牧草资源,对贵州部分野生草本植物种质资源的遗传多样性进行研究。根据模式植物拟南芥18SrRNA基因序列设计特异性引物,对贵州大学农场试验田自然生长的19种野生草本植物的18SrRNA基因序列进行扩增、测序、构建进化树。结果表明:将获得的1 000bp左右的DNA片段测序进行同源比对,共找到2 280个碱基变异位点,分布在8个区段。据各样本18SrRNA基因的遗传距离构建进化树推测,菊科、苋科和藜科之间存在较近的遗传相似性,豆科中三叶草属与豌豆属之间有较近的遗传距离。
In order to explore forage resource,the genetic diversity of 18 S rRNA gene in 19 kinds of wild herb germplasms were investigated,which were collected from the farm of Guizhou Unversity.The results showed that about 1000 bp fragments of 18 S rRNA genes were amplificated using specific primers based on the gene of Arabidopsis thaliana.After sequencing and homologous comparison,a total of 2 280 nucleotides were found out to be polymorphim sites.Phylogenetic tree of each family were constructed by similarity of 18S rRNA gene.The molecular classification of 19 kinds of wild herbs was consistent with its category based on morphological characteristics.Furthermore,the molecular classification could be useful to distinguish those similar species in morphology, and the genetic data suggested a close genetic relationship in three families,Compositae,Amaranthaceae and Chenopodiaceae.Trifolium and Pisum might share a high genetic similarty with each other.
出处
《贵州农业科学》
CAS
北大核心
2014年第2期12-17,共6页
Guizhou Agricultural Sciences
基金
贵州省科技创新人才团队
贵州省动物遗传育种与生物技术科技创新人才团队(黔科合人才团队2009-4006)
贵州省农业科技攻关项目"牧草基因资源发掘及种子分子鉴别关键技术研究与应用"(黔科合NY字2009-3082)
关键词
野生植物
种质资源
遗传多样性
遗传相似性
18S rRNA
wild plants
germplasm
18S rRNA
genetic diversity
genetic similarity
