摘要
利用中国被毛孢虫草菌液体深层发酵培养得到的菌丝体,通过正交试验优化得到了虫草多糖的提取工艺条件为:提取温度100℃,提取时间120 min,料液比1∶15,提取次数4次。粗多糖经Sevag法脱蛋白、透析袋透析、DEAE-cellulose离子交换和Sephadex G-100柱层析纯化后,得到3种多糖(命名为HSP-1、HSP-2和HSP-3)。经紫外扫描、Sephacryl S-300 HR柱层析检测确定HSP-1和HSP-2为均一组分,HSP-3为非均一组分。苯酚-硫酸法测得HSP-1和HSP-2的含糖量分别为89.42%和85.63%。Sephacryl S-300 HR柱层析测得HSP-1和HSP-2的相对分子质量分别为1.7×104Da和9.8×103Da。GC-MS测得HSP-1的单糖组成及比例为:D-葡萄糖∶D-甘露糖∶D-半乳糖=4.522∶1∶1.378,HSP-2的单糖组成及比例为:D-葡萄糖∶D-甘露糖∶D-半乳糖∶D-阿拉伯糖=2.687∶4.591∶1∶0.212。红外光谱测定结果显示HSP-1和HSP-2都含有α型糖苷键。
The mycelia were acquired by liquid submerged fermentation with Hirsutella sinensis. The extraction conditions were optimized through orthogonal test as follows： the extraction temperature was 100 ℃ , the extraction time was120 min, and the ratio of solid to liquid was 1： 15, for four times extraction. The crude polysaccharide was stepwise purified by Sevag deproteinization, dialysis, DEAE cellulose Ion exchange and Sephadex G-100 column chromatography, and three kinds of polysaccharides （named HSP-1, HSP-2, and HSP-3） were obtained. After ultraviolet scanning and Sephacryl S-300 HR column determination, HSP-1 and HSP-2 were regarded as homogeneous components while HSP-3 was inhomogeneous. The results from Phenol-sulfuric acid determination showed that the contents of HSP-1 and HSP-2 were 89.42% and 85.63% , respectively. The average molecular weights of HSP-1 and HSP-2, which were determined by Sephacryl S-300 HR column chromatography, were estimated to be 1.7 ×10^4 Da and 9. 8 × 10^3 Da, respectively. The results of GC-MS showed that monosaccharide compositions of HSP-1 contained D-glucose, D-mannose and D-galactose with molar ratio of 4. 522：1 ： 1. 378, while monosaccharide compositions of HSP-2 were D-glucose, D-mannose, D-galactose and D-arabinose with molar ratio of 2. 687： 4. 591 ： 1 ： 0. 2121. The FT-IR detection showed that the a-configuration glycosylic bond was contained in both HSP-land HSP-2.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2014年第3期222-226,共5页
Food and Fermentation Industries
基金
国家重点基础研究发展计划(973计划)基金项目(2012CB721006)
高等学校博士学科点专项科研基金(20110074110015)
关键词
中国被毛孢
冬虫夏草
多糖提取
纯化
理化性质
hirsutella sinensis, cordyceps sinensis, polysaccharide extraction, purification, physicochemical properties
