摘要
目的：建立东风桔总生物碱的含量测定方法并优选其提取工艺。方法：采用酸性染料比色法测定东风桔总生物碱含量，选择Buxifoliadine-B为指标成分，检测波长410 nm。以总生物碱提取量为指标，通过正交试验考察乙醇体积分数、乙醇用量、回流时间及提取次数对提取工艺的影响。结果：Buxifoliadine-B含量测定的线性范围0.034-0.34 g·L^-1，加样回收率（99.16±1.81）%。最佳提取工艺为加10倍量80%乙醇回流提取2次，每次1.5 h；总生物碱提取量4.88 mg·g^-1。结论：建立的东风桔总生物碱含量测定方法稳定可靠，优选的提取工艺方法合理可行。
Objective： To establish a determination for total alkaloids in Atalantia buxifolia and optimize its extraction process. Method： The content of total alkaloids was determined by acid dye-colorimetry,taking Buxifoliadine-B as index ingredient,detection wavelength was set at 410 nm.Effects of ethanol concentration,ethanol volume,extracting time and times on extraction amount of total alkaloids were investigated by orthogonal test. Result： The content of Buxifoliadine-B had a good linear relationship within the range of 0.034-0.34 g·L^-1,recovery was （99.16±1.81）%.Optimum extraction process was reflux extracted twice with 10 times the amount of 80% ethanol for 1.5 h per time;Under these conditions,extraction amount of total alkaloids was 4.88 mg·g^-1. Conclusion： This determination for total alkaloids in A. buxifolia and optimized extraction process were reasonable and practicable.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第7期40-42,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81202885)
国家"十二五"重大新药创制项目(2011zx09102-007-03)
广东省科技计划项目(2010B0307000444)
广州市科技计划项目(12A062131631)
广东省教育厅高层次人才项目(2010)
关键词
东风桔
总生物碱
酸性染料比色法
正交试验
提取工艺
Atalantia buxifolia
total alkaloids
acid dye-colorimetry
orthogonal test
extraction process
