摘要
目的:建立反相高效液相色谱法测定三七丹胶囊中人参皂苷Rb1、人参皂苷Rg1和三七皂苷R1的含量测定方法。方法:采用Hibar ODS C18(4.6mm×250mm,5μm)色谱柱,流动相为乙腈-水,梯度洗脱,流速1.0ml·min-1,柱温30℃,检测波长203nm。结果:人参皂苷Rb1、人参皂苷Rg1及三七皂苷R1分别在0.5246～5.2464μg(r=0.9997)、0.6792～6.7920μg(r=0.9998)和0.2542～2.5424μg(r=0.9997)范围内线性关系良好,平均回收率分别为人参皂苷Rb199.12%(RSD=0.61%,n=6)、人参皂苷Rg197.50%(RSD=1.63%,n=6)、三七皂苷R197.43%(RSD=1.04%,n=6)。结论:该方法定量简单、准确、重复性好,可作为三七丹胶囊的质量控制方法。
Objective：To establish an RP-HPLC method for determination of Ginsenoside Rbl,Ginsenoside Rgt and Notoginsenoside Ri in Sanqidan Capsules. Methods： Hibar ODS C18 （4.6mm×250mm,5μm） column was used to determine Ginsenoside Rb1l, Ginsenoside Rg1 and Notoginsenoside R1, with the mobile phase consisting of acetonitrile-water, gradient elution, the volume of flow 1.0 mIJmin, column temperature 30~C and detection wavelength 203nm. Restlts：The linear ranges of Ginsenoside Rbt, Ginsenoside Rgt and Notoginsenoside Ri were 0.5246~5.24641xg（r=0.9997） ,0.6792-6.7920p^g（r=0.9998） and 0.2542-2.54241xg（r=0.9997）, respectively. The average recoveries of 99.12% （RSD =0.61% ,n =6）for Ginsenoside Rbl,97.50% （RSD=l.63% ,n=6） for Ginsenoside Rg1 and Notoginsenoside RI 97.43%（RSD=l.04%,n=6）was obtained. Conclusion：The method is simple, accurate and with good reproduccibility. It can be used for the quality control of Sanqidan Capsules.
出处
《北方药学》
2014年第3期2-3,共2页
Journal of North Pharmacy
