摘要
目的 探讨强化剂量阿托伐他汀对不稳定性心绞痛患者PCI围术期CD4 T淋巴细胞Sprouty2表达的影响.方法 选择入住我院不稳定性心绞痛患者70例,随机分为强化他汀组35例（术前80 mg/d,术后40 mg/d）和常规他汀组35例（手术前后均20 mg/d）.分别于PCI术前（入院时未服用他汀）、术后16～24 h用免疫磁珠法分选出CD4 T淋巴细胞,荧光定量PCR检测Sprouty2 mRNA表达；免疫印迹法检测Sprouty2蛋白表达；ELISA检测TNF-α及白细胞介素（IL） 10的表达.结果 与术前比较,强化他汀组术后Sprouty2 mRNA、Sprouty2蛋白表达、IL-10明显升高,TNF-α明显下降（P＜0.05）；常规他汀组术后Sprouty2 mRNA、IL-10虽有升高趋势,TNF-α虽有下降趋势,但差异均无统计学意义（P＞0.05）.直线相关分析显示,TNF-α与Sprouty2表达量呈负相关（r=-0.836,P＜0.01）；IL-10与Sprouty2表达量呈正相关（r=0.754,P＜0.05）.结论 强化剂量的阿托伐他汀可以通过上调CD4 T淋巴细胞Sprouty2的表达,升高IL-10,降低TNF-α,从而减少PCI术后心肌的炎性反应.
Objective To study the effect of intensive atorvastatin on expression of Sprouty2 in unstable angina pectoris patients on perioperative PCI.Methods Seventy unstable angina pectoris patients admitted to our hospital were randomly divided into intensive atorvastatin dose treatment group and routine atorvastatin dose treatment group （35 in each group）.Patients in intensive atorvastatin dose treatment group received 80 mg atorvastatin/d and those in routine atorvastatin dose treatment group administered 20 mg atorvastatin/d.Peripheral blood samples were taken before PCI and 16-24 h after PCI.CD4T cells were isolated with a magnetic cell sorting system.Expression of Sprouty2,TNF-α and IL-10 was detected by RFQ-PCR,Western blot and ELISA,respectively.Results The Sprouty2 mRNA and protein and IL-10 expression levels were significantly higher while the TNF-α expression level was significantly lower in intensive atorvastatin dose treatment group after PCI than before PCI （P〈0.05）.Linear correlation analysis showed that the TNF-α expression level was negatively related with the Sprouty2 protein expression level while the IL-10 expression level was positively related with the Sprouty2 protein expression level （r=-0.836,P〈0.01 ;r=0.754,P〈0.05）.Conclusion Intensiv atorvastatin increases the IL-10 expression level and decreases the TNF-α expression level by upregulating the Sprouty2 expression level,thus reducing the myocardial inflammatory reactions after PCI.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2014年第3期243-247,共5页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
国家自然科学基金(81260042)
广西硕士研究生科研创新项目(YCSZ2013033)
