摘要
目的 观察血管内皮生长抑制因子（VEGI）及其相关因子在糖尿病（DM）大鼠外周血、玻璃体液及视网膜组织中的表达，探讨VEGI在糖尿病视网膜病变（DR）发病机制中的作用。方法 70只6周龄雄性Wistar大鼠，随机分为空白对照组（10只），DM 1、3、6个月组（各20只）。DM大鼠模型建立后，分别于1、3、6个月时取大鼠外周血、玻璃体液、全眼球。酶联免疫吸附测定法检测肿瘤坏死因子样配体1/血管内皮生长抑制因子251（TL1A/VEGI 251）、血管内皮生长因子（VEGF）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）在血清及玻璃体液浓度，石蜡切片免疫组织化学法检测各因子在视网膜组织中的表达，苏木素伊红（HE）染色评价各组大鼠DR进程。比较空白对照组和DM实验组间大鼠血清、玻璃体液及视网膜组织中TL1A、VEGF、TNF-α、IL-1β的表达差异。结果 分析采用单因素方差分析、独立样本t检验和最小显著差法检验。结果 空白对照组、DM 1、3、6个月组大鼠血清TL1A浓度分别为（92.09±2.05）、（118.36±8.30）、（85.90±7.51）、（78.90±4.88） ng/L，组间血清TL1A浓度比较，差异有统计学意义（F=77.405，P＜0.05）。从空白对照组到DM 1、3、6个月组，大鼠血清TNF-α、IL-1β浓度均呈升高趋势，4组间血清TNF-α、IL-1β浓度比较，差异有统计学意义（F=3.508、15.416，P＜0.05）。VEGF浓度在DM 1个月时升高，DM 3个月时下降，DM 6个月时再次升高，但4组间VEGF浓度比较，差异无统计学意义（F=1.242，P＞0.05）。各组大鼠玻璃体液TL1A浓度分别为（91.50±8.18）、（67.03±6.74）、（47.44±4.92）、（46.01±4.62） ng/L，组间TL1A浓度比较，差异有统计学意义（F=114.777，P＜0.05）。从空白对照组到DM 1、3、6个月组，大鼠玻璃体液VEGF、TNF-α、IL-1β浓度均呈升高趋势，4组间VEGF、TNF-α、IL-1β比较，差异有统
Objective To observe the expression of vascular endothelial growth inhibitor （VEGI,TL1A）, vascular endothelial growth factor （VEGF）, tumor necrosis factor-α （TNF-α） and interleukin-1β （IL-1β） in diabetes rats′ serum, vitreous and retina, and discuss the role of VEGI in the pathogenesis of diabetic retinopathy （DR）. Methods A total of p70 adult male Wistar rats were randomly divided into 4 groups, the control group （10 rats）, the diabetes mellitus （DM） 1 month group （20 rats）, the DM 3 month group （20 rats） and the DM 6 month group （20 rats）. Cytokines of serum and vitreous were determined by enzyme-linked immunosorbent assay （ELISA）, and the concentrations of the cytokines in the retina were determined by immunohistochemistry on paraffin retinal sections. Hematoxylin-eosin （HE） staining of retina was used to estimate the pathological change of DR. The results were analyzed by one-way analysis of variances, independent samplest-test and LSD test. Results The serum TL1A levels of the control group, the DM 1 month group, the DM 3 month group and the DM 6 month group rats were （92.09±2.05）, （118.36±8.30）, （85.90±7.51） and （78.90±4.88） ng/L respectively, the level of TL1A in serum of the DM 1 month group, the DM 3 month group and the DM 6 month group were significantly lower than that of the control group （F=77.405, P〈0.05）. The concentration of serum TNF-α and IL-1β increased after DM model was established （F=3.508, 15.416;P〈0.05）; the VEGF level in serum showed no difference between the groups （F=1.242, P〉0.05）. The vitreous TL1A levels of the control group, the DM 1 month group, the DM 3 month group and the DM 6 month group were （91.50±8.18）, （67.03±6.74）, （47.44±4.92） and （46.01±4.62） ng/L respectively, every DM groups showed significant difference with the control group （F=114.777,P〈0.05）; VEGF level in vitreous increased from 1 month after DM model was established （F=8.816,P〈0.05）; TNF
出处
《中华眼底病杂志》
CAS
CSCD
北大核心
2014年第2期180-186,共7页
Chinese Journal of Ocular Fundus Diseases
