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大肠杆菌中从头合成白藜芦醇途径的设计及优化 被引量:6

Pathway Assembly and Optimization in E. coli for de Novo Biosynthesis of Resveratrol
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摘要 白藜芦醇是一种极具药用价值的植物源芪类化合物。为了在E.coli实现白藜芦醇的从头合成,构建了由酪氨酸解氨酶(TAL),香豆酸-CoA合成酶(4CL)和白藜芦醇合成酶(STS)组成的非天然合成途径。经3天发酵后,白藜芦醇产量仅为2.67 mg/L,而其中间体香豆酸的积累达到了95.64 mg/L。为了进一步改善异源途径的效率,对4CL和STS模块采取融合表达、高拷贝表达及启动子工程改造的策略,最终使白藜芦醇产量提高到了9.6倍,达到了25.76 mg/L,同时香豆酸的积累减少到了20.38 mg/L。这些研究结果为更高效白藜芦醇从头合成工程菌的构建及最终实现白藜芦醇的微生物大规模生产奠定了基础。 Resveratrol is an important plant stilbene with considerable pharmaceutical values. To achieve the de novo biosynthesis of resveratrol in E. coli, a heterologous resveratrol biosynthetic pathway consisting of tyrosine ammonia lyase (TAL), coumaroyl-CoA synthase (4CL) and stilbene synthase (STS) was constructed. The engineered strain only produced 2.67 mg/L resveratrol after 3 clays cultivation. To improve the efficiency of heterologous pathway, the 4CL and STS modules were further engineered using strategies of fusion expression, high-copy expression and promoter engineering. Eventually, the yield of resveratrol reached 25.76 mg/L with a 9.6-fold improvement compared with the initial strain. The useful information for the construction of more efficient recombinant resveratrol producer was provided, and it laid a foundation for the large-scale production of resveratrol through microbial fermentation.
出处 《中国生物工程杂志》 CAS CSCD 北大核心 2014年第2期71-77,共7页 China Biotechnology
基金 国家“973”计划(2012C13721104) 国家自然科学基金(31170101,31100073) 中国科学院知识创新项目(ICSCXZ-EW-J-12)资助项目
关键词 大肠杆菌 白藜芦醇 融合表达 高拷贝表达 启动子工程 Escherichia coli Resveratrol engineering Fusion expression High-copy expression Promoter
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  • 1King R E, Bomser J A, Min D B. Bioactivity of resveratrol. Comprehensive Reviews in Food Science and Food Safety, 2006, 5(3) :65-70. 被引量:1
  • 2Donnez D, Jeandet P, C16ment C, et al. Bioproduction of resveratrol and stilbene derivatives by plant cells and microorganisms. Trends in Bioteehnology, 2009, 27 ( 12 ) : 706- 713. 被引量:1
  • 3Xu P, Bhan N, Koffas M A. Engineering plant metabolism into microbes: from systems biology to synthetic biology. Current Opinion in Bioteehnology, 2013, 24(2) :291-299. 被引量:1
  • 4Kirby J, Keasling J D. Metabolic engineering of microorganisms for isoprenoid production. Natural Product Reports, 2008, 25 (4) :656-661. 被引量:1
  • 5Wang Y, Chen S, Yu O. Metabolic engineering of flavonoids in plants and microorganisms. Applied Microbiology and Biotechnology, 2011, 91 (4) :949-956. 被引量:1
  • 6Lim C G, Fowler Z L, Hueller T, et al. High-yield resveratrol oroduction in engineered Escherichia coli. Applied and Environmental Microbiology, 2011, 77 ( 10 ) :3451-3460. 被引量:1
  • 7Sydor T, Schaffer S, Boles E. Considerable increase in resveratrol production by recombinant industrial yeast strains with use of rich medium. Applied and Environmental Microbiology, 2010, 76 (10) :3361-3363. 被引量:1
  • 8Shin S Y, Han N S, Park Y C, et al. Production of resveratrol from p-coumaric acid in recombinant Saccharomyces cerevisiae expressing 4-coumarate:coenzyme A ligase and stilbene synthase genes. Enzyme and Microbial Technology, 2011,48 (1) :48-53. 被引量:1
  • 9Watts K T, Lee PC, Schmidt-Dannert C. Biosynthesis of plant- specific stilbene polyketides in metabolically engineered Escherichia coli. BMC Biotechnology, 2006, 6:22. 被引量:1
  • 10Beekwilder J, Wolswinkel R, Jonker H, et al. Production of resveratrol in recombinant microorganisms. Applied and Environmental Microbiology, 2006, 72(8) :5670-5672. 被引量:1

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