摘要
试验对延边黄牛甲状腺球蛋白(thyroglobulin,TG)基因进行克隆测序,采用PCR-RFLP方法进行多态性检测,并与生长性状进行了关联分析。结果表明,在TG基因第48外显子上发现C218T和A430G2个突变位点,其中C218T位点的不同基因型与胸宽存在显著相关(P<0.05),TT基因型个体胸宽显著高于CT基因型个体(P<0.05)。A430G位点的不同基因型与体重、尻长和坐骨端宽存在显著相关(P<0.05),GG基因型个体的体重显著高于AA基因型个体(P<0.05),AA和AG基因型个体的尻长显著高于GG基因型(P<0.05),AG基因型个体的坐骨端宽显著高于GG基因型(P<0.05)。其他性状在2个位点的不同基因型间差异不显著(P>0.05)。因此,C218T和A430G位点可能是影响延边黄牛生长性状的分子标记。
The SNPs of Yanbian Yellow cattles thyroglobulin （TG） gene were detected by cloning and sequencing, con- firmed by technique of PCR-RFLP,and studied the association of polymorphisms of TG gene with growth traits. The results showed that there were C218T and A430G two mutations in the exon 48 in TG gene of Yanbian Yellow cattle. The association analysis showed that the chest breadth with TT genotype in C218T locus was significantly higher than that of CT genotype （P〈0.05）. A430G locus polymorphism significantly associated with the body weight, rump length and hucklebone width（P% 0.05）. The body weight of GG genotype was higher than AA genotype（P〈0.05）, the rump length of AA and AG genotypes were higher than GG genotype（P〈0.05） ,and the hucklebone width of AG genotype was higher than GG genotype within pop- ulation（P〈0.05）. In a word, C218T and A430G SNPs might be a good DNA marker to be used in MAS for growth traits.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第3期188-193,共6页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目"延边黄牛肉质相关功能基因研究"(31160442)
关键词
延边黄牛
甲状腺球蛋白基因
多态性
生长性状
. Yanbian Yellow cattles
thyroglobulin gene
polymorphism
growth traits
