摘要
目的:通过检测ER、PR、Ki-67在子宫腺肌病(AM)在位内膜及腺肌病病灶区的表达,检测其血浆CA125水平,探讨各因子与子宫腺肌病的关系。方法:采用EnVision二步法检测68例子宫腺肌病患者在位内膜、腺肌病病灶区及15例对照组在位内膜中ER、PR、Ki-67的表达情况,采用VAS数字评分法评定腺肌病患者疼痛程度,光化学发光法检测其血浆CA125水平,经阴道超声测量子宫各径线并计算体积,采用AFS评分法评定盆腔粘连情况,比较它们之间的关系。结果:ER、PR的表达为AM病灶区<在位内膜及对照组(P<0.05),Ki-67的表达为AM区域>在位内膜及对照组(P<0.05),在位内膜及对照组ER、PR、Ki-67的表达无统计学差异(P>0.05)。AM组织中ER、PR与Ki-67有相关性(P<0.05);CA125与疼痛程度、子宫体积、AFS评分呈中度相关(P<0.05)。结论:AM病灶区ER、PR与Ki-67的表达具有相关性,但均无周期性变化,提示AM病灶区内膜细胞增殖活动在一定程度上不受卵巢激素的调节,可能异位内膜与在位内膜的生长环境、增殖分化机制存在一定差异;CA125与疼痛程度、子宫体积、ASRM评分相关,它的检测对AM的辅助诊断和观察病情进展情况有重要的临床意义。
Objective： To detect the expressions of estrogen receptor （ER）, progesterone receptor （PR）, and Ki -67 in eutopic endometrium and focal area, plasma CA125 level of patients with adenomyosis, explore the relationship between ER, PR, Ki -67, CA125 and adenomyosis. Methods： EnVision two - step method was used to detect the expressions of ER, PR, and Ki - 67 in entopic endometrium and focal area of 68 patients with adenomyosis and 15 cases in control group; VAS numeric rating scale was used to evaluate the degree of pain in patients with adenomyosis; photochemical luminescence method was used to detect plasma CA125 level; transvaginal ultrasound was used to measure uterine radial lines and calculate uterine volume, AFS grading method was used to assess pelvic adhesion situation and compare the relationship between them. Results： The expression levels of ER and PR in focal area of adenomyosis group was statistically significantly lower than those in eutopie endometrium of adenomyosis group and control group （ P 〈 0. 05 ） , the expression level of Ki - 67 in focal area of adenomyosis group was statistically significantly higher than those in eutopic endometrium of adenomyosis group and control group （P 〈 0. 05 ）, there was no statistically significant difference in the expression levels of ER, PR, and Ki - 67 between eutopic endometrium of adenomyosis group and control group （P 〉 0. 05 ） ; there was a correlation between ER, PR and Ki - 67 in adenomyosis group （ P 〈 0. 05 ） ; CA125 was moderately correlated with painful degree, uterine volume, and AFS score （P 〈 0. 05 ） . Conclusion ： There is a correlation between ER, PR and Ki -67 in adenomyosis in focal area of patients with adenomyosis, but there isn＇t periodical change, which indicates that proliferation of endometrial cells in focal area of patients with adenomyosis cant be regulated by ovarian hormones to a certain degree, there are a certain differences in growing environment, proliferation and differentiation mec
出处
《中国妇幼保健》
CAS
北大核心
2014年第9期1405-1408,共4页
Maternal and Child Health Care of China
基金
新疆兵团边境地区妇女多发病诊治适宜技术示范应用项目〔2013GS650104〕
