摘要
以芦竹为供试植物,通过盆栽试验研究在铜、锌复合胁迫下,芦竹生理生化特性的变化,对铜锌吸收积累的规律和4种土壤酶活性的影响。结果表明,在较低复合浓度Cu2+为100mg/kg,Zn2+为100mg/kg时,芦竹植株开始出现伤害症状;随铜、锌复合浓度的增大,叶片中叶绿素含量不断下降,CAT和SOD活性先上升后下降,POD活性持续下降,MDA和脯氨酸含量及细胞质膜透性均随铜锌复合浓度的增加而上升;根部是重金属累积的主要部位,芦竹中重金属铜、锌含量的分布规律均为根(地下部分)>茎、叶(地上部分),根和地上部分的Cu、Zn累积浓度随着Cu、Zn添加浓度的升高不断上升;低浓度Cu2+为100mg/kg,Zn2+为100mg/kg对土壤中蔗糖酶的活性有一定的促进作用,当复合浓度达到Cu2+为200mg/kg,Zn2+为200mg/kg后,蔗糖酶的活性随复合浓度的上升呈下降趋势,其余3种酶的活性始终随复合浓度的升高而降低。研究表明,芦竹对铜锌复合污染土壤具有一定耐受力。
Using Arundo dona：c as experimental plant, through the pot experiments, the physiological, biochemical characteristics, accumulation and soil enzyme activities under combined stress of Cu and Zn were studied. The results showed as follows： Under combined stress of Cu and Zn, the plants showed serious apparent injury at lower concentration of Cu2＋ 100 mg/kg and Zn2＋ 100 mg/kg. With the increase of complex concentration of Cu and Zn, the chlorophyll content and the activity of POD declined constantly, and the activities of CAT and SOD increased firstly then reduced, MDA content, proline content and cell membrane permeability increased with increasing concentration. Under combined treatment of Cu and Zn, according to the experiment results, root was the main part of heavy metal accumulation, the distribution of Cu and Zn in Arundo donax was higher in roots（underground part） than in stems and leaves（aboveground part）, and accumulation of roots and aboveground part rose with increasing concentration. The activity of invertase promoted at Cu2＋ 100 mg/kg and Zn2＋ 100 mg/kg, when the concentration reached Cu2＋ 200 mg/kg and Zn2＋ 200 mg/kg, it declined with increasing concentration. The activities of other three soil enzymes decreased gradually with increasing concentration. Results also indicated that Arundo donax was a tolerant plant species to copper and zinc.
出处
《水土保持学报》
CSCD
北大核心
2014年第1期276-280,288,共6页
Journal of Soil and Water Conservation
基金
安徽高校省级自然科学研究项目(KJ2012B219)
安徽省高校青年教师资助计划项目(2008jq1182)
安徽省高校自然科学基金重点项目(kj2011A129)
安徽省自然科学基金项目(11040606M77)
关键词
芦竹
铜
锌
生理生长指标
富集
土壤酶活性
Arundo donax
Cu and Zn
growth and physiological index
accumulation
soil enzyme activities
