摘要
草莓斑驳病毒(SMoV)和草莓轻型黄边病毒(SMYEV)分布较广,危害较重。应用CTAB法提取草莓总RNA,以优质的草莓总RNA为模板,结合扩增内标的检测体系,建立了SMoV和SMYEV的多重RT-PCR检测体系,可以对草莓田间植株和试管苗进行快速稳定的病毒检测。
Strawberry mottle virus（SMoV） and Strawberry mild yellow edge virus（SMYEV）, which are found world-wide, can cause serious crop losses .Total RNA for reverse transcriptase-polymerase chain reaction （ RT-PCR） was extracted from strawberry with CTAB method .A multiplex RT-PCR assay was used for simultaneous detecting SMoV and SMYEV based on ndh B gene as the internal control .Both field plants and test-tube plantlets of strawberries were detected effectively .
出处
《浙江农业学报》
CSCD
北大核心
2014年第1期105-109,共5页
Acta Agriculturae Zhejiangensis
基金
农业科技成果转化资金项目(SQ2012ECC220008)
关键词
草莓病毒
内标
病毒检测
多重RT-PCR
strawberry virus
internal control
multiplex RT-PCR
virus detection
