摘要
目的:观察清热祛湿活血方对高糖高脂饮食诱导的非酒精性脂肪肝模型大鼠血浆脂联素(APN)、脂肪组织二硫键A样氧化还原酶蛋白(DsbA-L)等指标的影响。方法:将30只大鼠随机分为观察空白对照组(A组)、模型对照组(B组)和中药组(C组),每组各10只。造模成功后,C组取中药煎剂按每日10 mL·kg-1剂量灌胃,A组和B组用相同体积的生理盐水灌胃。观察各组大鼠治疗前、治疗后体质量的变化和治疗后肝指数变化;光镜观察大鼠肝脏病理学变化。结果:中药组体质量与模型对照组治疗前比较,差异无统计学意义(P>0.05);中药组大鼠体质量与空白对照组治疗前比较,差异具有统计学意义(P<0.01);与模型对照组和空白对照组治疗后比较,中药组大鼠体质量及肝指数显著低于模型对照组,;血浆APN和脂肪组织DsbA-L的表达水平显著高于空白对照组和模型对照组,差异均有统计学意义(P<0.01);光镜下观察模型对照组肝细胞脂肪变性明显而广泛,肝细胞结构紊乱,而中药组肝组织结构正常。结论:清热祛湿活血方能降低非酒精性脂肪肝模型大鼠体质量及肝指数,显著改善肝组织损害,可能与该方能够显著促进大鼠脂肪组织DsbA-L的表达,进而促进脂联素的表达有关。
Objective： To observe the influence of Qingre Qishi Huoxue fonnula on Plasma adiponectin （ APN） and DshA - L expression of rats with nonalcoholic fatty liver caused by high - glucose and high - fat diet. Methods ：30 rats were randomly divided into three groups： Blank control group（ A group） , model control group （ B group） ,and Chinese medicine group（ C group） ,with 10 in each group. Mter succeeding in building the model, each rat in C group was given lOmVkg Chinese medicine decoction each day by gastric perfusion, and each rat in A group and B group was given the same amount of nonnal saline in the same way. The changes of body quality before and after treatment and changes of liver index after treatment were observed;Each rats liver pathologic changes were also observed through light microscope. Results： Comparing Chinese medicine group with model control group before treatment, the body quality difference had no statistical significance （ P 〉 0. 05 ）. Comparing Chinese medicine group with blank control group before treatment, the body quality difference had statistical significance （ P 〈 0. 01 ）. Compared with model control group and blank control group after treatment,a rats body quality and liver index in Chinese medicine group were clearly lower than those in model control group; And APN and DsbA - L expression level were much higher than those in blank control group and model control group. The differences had statistical significance （P 〈 O. 01 ）. Through light microscope, liver cell fat change in model control group was clear and wide, and liver cell construction was in disorder, while liver cell construction in Chinese medicine group was normal. Conclusion： Qingre Qishi Huoxue F onnula can reduce body quality and liver index of model rats with nonalcoholic fatty liver ,and clearly improve liver tissue damage. Probably this is because this fonnula can clearly improve rats ＇fat tissue DsbA - L expression level and APN.
出处
《河南中医》
2014年第3期409-411,共3页
Henan Traditional Chinese Medicine
基金
广西自然科学基金项目(编号:2012GXNSFAA053098)
