摘要
目的:优选聚酰胺树脂分离纯化裸花紫珠总黄酮的工艺条件。方法:以总黄酮含量为指标,利用静态-动态吸附-洗脱试验考察聚酰胺树脂纯化总黄酮的行为,通过单因素试验考察饱和吸附量、pH、洗脱溶剂用量等工艺参数。结果:最佳精制工艺参数为上样液质量浓度0.073 g·mL-1,上样量2 BV,上样液pH 3.0,加水3 BV洗脱杂质,用80%乙醇3 BV洗脱,收集乙醇洗脱液;总黄酮纯度由5.6%提高至42.3%,平均保留率89.6%(RSD 3.56%)。结论:采用聚酰胺树脂纯化裸花紫珠总黄酮的效果良好,为裸花紫珠新制剂的开发提供实验依据。
Objective：To optimize purification technology of total flavonoids from Callicarpa nudiflora by polyamide resin.Method：With the content of total flavonoids as index,adsorption and elution behavior of polyamide resin for total flavonoids was investigated by stastic-dynamic adsorption-elution test,single factor tests were adopted to optimize purification technology for total flavonoids taking saturated adsorption,pH,elution volume and other parameters.Result：Optimum purification technology parameters were as follows：the concentration of sample solution 0.073 g ·mL-1,sample volume of 2 BV,pH of sample solution 3.0,eluted impurities with 3 BV water,then eluted with 3 BV of 80％ ethanol; Purity of total flavonoids was up to 42.3％ from 5.6％,average retention rate was 89.6％ （RSD 3.56％）.Conclusion：Purification effect of total flavonoids from C.nudiflora by polyamide resin was feasible,and this study could provide experimental basis for development of new formulations from C.nudiflora.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第6期23-25,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
海南省重点科技计划项目(ZDXM20120095)
浙江省公益技术研究社会发展项目(2012C23082)
海南医学院科研培育基金项目(HY2010-011)
关键词
裸花紫珠
总黄酮
聚酰胺树脂
单因素试验
静态-动态吸附试验
纯化工艺
Callicarpa nudiflora
total flavonoids
polyamide resin
single factor test
static-dynamic adsorption test
purification technology
