摘要
目的探讨改良支撑材料小鼠颅颌面器官体外培养的可行性及适宜条件。方法分离出生后0.5天(PN0.5)的C57BL小鼠颅颌面部分器官(下颌磨牙、下颌下腺、舌),在由有机树脂片作为支撑物的6孔板中体外培养3天,组织学切片HE染色,观察所培养器官的生长发育情况和形态学变化,并且与PN0.5和PN3.5小鼠的相应器官进行组织学比较。结果相对于PN0.5的颅颌面器官结构,器官培养3天后下颌磨牙、下颌下腺和舌等器官体积增大,并有不同程度的发育,细胞有不同程度的增殖和分化。体外3天培养器官的发育要落后于PN3.5的实际生长。结论本研究初步探索并建立了小鼠颅颌面器官树脂支撑物体外培养系统。
Objective To construct a modified system of culturing the mice organ in vitro. Methods The tonge, submandibular gland ,lower molar from PN0. 5 C57BL mice were dissected. All samples were cultured at CO2 incubator for 3 days in the modified organ culture system which contained 6-well plates, resin supporter and culture medium and then processed into paraffin-embedded serial sections for HE staining. The tissue structures after culture were compared with structures of PN0. 5 and PN3.5 mice. Results After 3 days culture in vitro, the mice craniofacial organs all survived. They t^ew and developed well. Histology showed that some cells proliferated and differentiated and the organs enlarged. However, the development of the craniofacial structures in the organ culture system was delayed compared with PN 3.5 mice. Conclusion A modified culture system for craniofacial structure with resin supporter was successfully set up.
出处
《北京口腔医学》
CAS
2014年第1期17-21,共5页
Beijing Journal of Stomatology
基金
国家自然基金(81170943)
北京市自然基金(7122051)
北京市卫生系统高层次卫生技术骨干人才培养计划(2011-3-008)
教育部留学回国人员科研启动基金项目
