摘要
目的探讨不同分散度的沙漠尘对大鼠肺损伤和巨噬细胞分泌肿瘤坏死因子(TNF-α)、白细胞介素8(IL-8)和一氧化氮(NO)的影响。方法沙漠尘样品采自内蒙古腾格里沙漠。120只Wistar雄性大鼠随机分为阴性对照组、粒径≤2.5μm沙漠尘组、≤10μm沙漠尘组和SiO2阳性对照组,每组30只。将浓度均为60 mg/ml的SiO2、粒径≤2.5和≤10μm沙漠尘混悬液,按100 mg/kg染毒剂量、1.67 ml/kg染毒体积,采用气管内灌注法染毒两次,间隔15 d,阴性对照组灌注生理盐水。每组分别在染尘后1、3、6个月处死10只,取肺组织进行病理学检查。对原代培养的大鼠肺泡巨噬细胞染毒24 h后,采用四甲基偶氮唑盐(MTT)比色法测定细胞毒性,用酶联免疫法检测TNF-α和IL-8,用Griess重氮化反应法检测NO。结果粒径≤2.5、≤10μm组大鼠染毒后1个月时肺泡间隔增厚、细胞浸润,3个月时肺组织部分区域出现纤维化,6个月时出现较明显的尘肺结节样病变,且粒径≤2.5μm组纤维化面积相对较大。粒径≤2.5、≤10μm的沙漠尘作用巨噬细胞24 h后,随着染毒剂量的增加,其细胞毒性逐渐增强,最高剂量组的细胞存活率分别为72.42%和81.76%;诱导细胞分泌TNF-α、IL-8和NO量明显增加,染毒剂量为100、200μg/ml时,各炎性因子分泌量高于阴性对照组,差异有统计学意义(P<0.05或P<0.01)。结论粒径≤2.5、≤10μm的沙漠尘均能诱导大鼠肺巨噬细胞分泌炎性因子TNF-α、IL-8和NO的量增加,可使染尘大鼠发生典型的尘肺结节性病变。
Objective To study the effects of desert dust with different dispersity on lung injury and secretion of tumor necrosis factor-α （TNF-α）, interleukin-8 （IL-8） and nitric oxide （NO） in macrophages of rats. Methods Desert dust was collected during sand storm in the Tengger area in Inner Mongolia. A total of 120 male Wistar rats were randomly divided into negative control group, particle size≤2.5 Iμm group, particle size ≤ 10 μm group and positive control group. The animal models were established by direct tracheal instillation of particle size≤ 2.5 μm and ≤ 10 μm of desert dust and silica into rat lungs. In the lth, 3 th, 6 th month of the establishment of animal model, ten rats were killed in each group at one time. Lung injury was tested by H.E staining. The primary alveolar maerophages were cultured and the levels of cytokines TNF-α and IL-8 were determined by using enzyme-linked immunity assay. The content of NO was determined by using Griess reagent. Results In the lth month,the lung tissue of rats were infiltrated by macrophages, lymphocytes and mononuclear cells in particle size≤2.5 μm group, particle size≤ 10μm group. In the 6th month,the lung tissue were infiltrated by typical pneumoconiosis nodules in particle size ≤2.5 μm group, particle size ≤10μm group. At concentrations of 50-200μg/ml, particle size≤ 2.5 μm and ≤ 10 μm of desert dust increased the TNF-α,IL-6 and IL-8 and NO in alveolar macrophages. At concentrations of 100, 200 μg/ml, the levels of cytokines TNF-α, IL-8 and content of NO were significantly higher than those of negative control group （P〈0.05, P〈0.01）. Conclusion The particles of size ≤2.5 μm and ≤ 10 μm from desert dust may induce the increased secretion of inflammatory cytokines TNF-α,IL-8 and NO in alveolar macrophages and lung tissue may be infiltrated by the typical pneumoconiosis nodular lesions in rats.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2014年第1期33-36,F0003,共5页
Journal of Environment and Health
基金
包头市医药卫生科技发展基金(2006-31)
