摘要
目的 研究 CD147与 MMPs(基质金属蛋白酶 )在肝癌细胞系中表达的相关性 .方法 流式细胞仪检测正常肝细胞 QZG及不同肝癌细胞系 (BEL - 740 2 ,QGY- 740 4,SMMC-772 1,HHCC和 HHCC- 92 0 4)中 CD147的表达 ;酶谱法分析其产生 MMPs的含量及活性 .结果 正常肝细胞株 QZG中CD147表达阴性 ,其余 5株肝癌细胞系 CD147表达阳性 ;MMP- 2和 MMP- 9在 QZG和肝癌细胞系中均表达 ,但在肝癌细胞系中表达明显增高 ;MMP- 9以酶原和活性形式表达 ,MMP- 2仅以酶原形式表达 ;统计学分析 CD147与 MMPs表达成正相关 .结论
AIM To study the relation between CD147 and the expression of MMPs in hepatoma cell lines. METHODS Expression of CD147 in human liver cell QZG and different hepatoma cell lines (BEL 7402, QGY 7404, SMMC 7721, HHCC, HHCC 9204) was investigated by flow cytometer. Zymograph was used to study the content and types of positive MMPs. RESULTS CD147 was positively presented in five hepatoma cell lines, but in normal liver cell was negative. MMP 2 and MMP 9 were detected by all the hepatoma cell lines and normal liver cell but the expression in hepatoma cell lines was obviously high. The relation between CD147 molecule and the expression of MMPs was a positive one. CONCLUSION CD147 could be used as a marker to judge the invasion and metastasis of hepatocellular carcinoma.
出处
《第四军医大学学报》
北大核心
2001年第3期217-219,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金重点项目资助 !(3 99890 0 2 )
